Vitrification of oocytes and in vitro produced bovine embryos exposed to cytochalasin B

  • Alceu Mezzalira Universidade do Estado de Santa Catarina, Centro de Ciências Agroveterinárias, Lages, SC
  • Arnaldo Diniz Viera Universidade Federal de Santa Maria, Centro de Ciências Rurais, Departamento de Clínica de Grandes Animais, Laboratório de Embriologia Animal, Santa Maria, RS
  • Dilmar Paulo Barbieri Universidade do Estado de Santa Catarina, Centro de Ciências Agroveterinárias, Lages, SC
  • Mariana Fernandes Machado Universidade do Estado de Santa Catarina, Centro de Ciências Agroveterinárias, Lages, SC
  • André Thaler Neto Universidade do Estado de Santa Catarina, Centro de Ciências Agroveterinárias, Lages, SC
  • Mari Lourdes Bernadi Universidade Federal do Rio Grande do Sul, Faculdade de Agronomia, Departamento de Zootecnia, Porto Alegre, RS
  • Carlos Antônio Mondino Silva Universidade Federal de Santa Maria, Centro de Ciências Rurais, Departamento de Clínica de Grandes Animais, Laboratório de Embriologia Animal, Santa Maria, RS
  • Mara Iolanda Batistella Rubin Universidade Federal de Santa Maria, Centro de Ciências Rurais, Departamento de Clínica de Grandes Animais, Laboratório de Embriologia Animal, Santa Maria, RS
Keywords: Bovine, Cytochalasin B, Embryos

Abstract

In this study, the influence of cytochalasin B on the survival and development after vitrification of oocytes and in vitro produced (IVP) bovine blastocysts was evaluated. In the Experiment I, 956 oocytes were matured for 22h and were immediately vitrified (Vitri treatment) or exposed for 15-20 minutes, to 7.5µg/ml (CB7.5Vitri treatment) or 45µg/ml (CB45Vitri treatment) cytochalasin B solutions, before vitrification. After 30 seconds of exposure to SV1 [400µl TCM-Hepes with 10% fetal serum (SF), 50µl ethylene glycol (EG) and 50µl DMSO], and 20 seconds to SV2 (300µl trehalose 1,0M + 20% SF, 100µl EG and 100µl DMSO) solutions, the oocytes were vitrified in Open Pulled Straws (OPS). The rewarming was performed at 37-38ºC in two steps of 5 minutes each, into 0.3 and 0.15M trehalose solutions, respectively. There was no difference (P>;0.05) in the cleavage and embryo rates between Vitri, Cito7.5Vitri and Cito45Vitri treatments, which were inferior to control group (P;0.05), which were lower than those observed in the Control group (P<0.05). The results show that, independently of dose studied (7.5 or 45mg/ml), cytochalasin B has not a beneficial effect to the vitrification of oocytes and IVP bovine blastocysts.

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Published
2002-01-01
How to Cite
Mezzalira, A., Viera, A., Barbieri, D., Machado, M., Thaler Neto, A., Bernadi, M., Silva, C., & Rubin, M. (2002). Vitrification of oocytes and in vitro produced bovine embryos exposed to cytochalasin B. Brazilian Journal of Veterinary Research and Animal Science, 39(5), 260-265. https://doi.org/10.1590/S1413-95962002000500008
Section
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