This article has no title in english

  • Maria Gabriela Tavares Rheingantz Universidade Federal de Pelotas, Centro de Biotecnologia, Pelotas, RS
  • Ligia Margareth Cantarelli Pegoraro Embrapa Clima Temperado, Pelotas, RS
  • Odir Antonio Dellagostin Universidade Federal de Pelotas, Centro de Biotecnologia, Pelotas, RS
  • Anita Mylius Pimentel Universidade Federal de Pelotas, Centro de Biotecnologia, Pelotas, RS
  • Mari Lourdes Bernardi Universidade Federal do Rio Grande do Sul, Faculdade de Agronomia, Departamento de Zootecnia, Porto Alegre, RS
  • João Carlos Deschamps Universidade Federal de Pelotas, Centro de Biotecnologia, Pelotas, RS
Keywords: In vitro fertilization, Sex ratio, Glucose, Percoll gradient, Swim-up

Abstract

In the bovine IVP system, it has been obtained a high percentage of male embryos. This experiment was carried ou to determine if the glucose presence in the culture medium affects the sex ratio of bovine embryos IVP from the IVF with spermatozoa prepared for the swim-up (S) or Percoll gradient (P) methods. After the IVM, the COCs had been divided in two groups and inseminated with spermatozoa prepared for one of the methods. The zygotes had been cultivated in medium with or without 5.56mM of glucose, totalizing 4 treatments: S-Glu, S+Glu, P-Glu and P+Glu, and 48h post-insemination, the embryos of each treatment had been sexed for the PCR method (n=845). The effect of glucose in the culture medium on sex ratio of embryos IVP from the two methods was similar (c² test), resulting in a percentage of males lower than 50% in the 2-C stage (S: 30.8%; P: 23.8%: P<0.01) and higher than 50% in the 8-C stage (S: 79.4%; P: 68.8%: P<0.01). These percentages were different (P<0.05) of the observed when the embryos were cultivated without glucose, as much in the 2-C stage (S: 48.5%; P: 41.5%) as in the 8-C stage (S: 62.5%; P: 50.8%). The glucose presence did not affect the sex ratio in the total of produced embryos (S: 56.7%; P: 49.0%), that was similar to the observed in the glucose absence (S: 55.7%; P: 46.2%). Therefore, the glucose exacerbated the difference in the development speed between the female and male embryos.

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Published
2004-02-01
How to Cite
Rheingantz, M., Pegoraro, L., Dellagostin, O., Pimentel, A., Bernardi, M., & Deschamps, J. (2004). This article has no title in english. Brazilian Journal of Veterinary Research and Animal Science, 41(1), 32-39. https://doi.org/10.1590/S1413-95962004000100006
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