Effects of sodium fluoride in gill epithelium of Guppy fish (Poecilia vivipara)

  • Leandro Breseghelo Universidade Federal de Goiás, Instituto de Ciências Biomédicas, Departamento de Morfologia, Laboratório de Comportamento Celular, Goiânia, GO
  • Márcia Pereira Cardoso Universidade Federal de Goiás, Instituto de Ciências Biomédicas, Departamento de Morfologia, Laboratório de Comportamento Celular, Goiânia, GO
  • Rodinelli Borges-de-Oliveira Universidade Federal de Goiás, Instituto de Ciências Biomédicas, Departamento de Morfologia, Laboratório de Comportamento Celular, Goiânia, GO
  • Marcelo Ferreira da Costa Universidade Federal de Goiás, Instituto de Patologia Tropical, Goiânia, GO
  • José Clecildo Bezerra Barreto Universidade Federal de Goiás, Instituto de Patologia Tropical, Goiânia, GO
  • Simone Maria Teixiera de Sabóia-Morais Universidade Federal de Goiás, Instituto de Ciências Biomédicas, Departamento de Morfologia, Laboratório de Comportamento Celular, Goiânia, GO
  • Áureo Tatsumi Yamada Universidade Estadual de Campinas, Instituto Biológico, Laboratório de Citoquímica, Campinas, SP
Keywords: Chloride cells, Fluorine, Gills, Mucous cells, Schistosomiasis

Abstract

Fluorine is an element much used by man and easily found in nature. Some studies have been carried out on the toxicity of fluorine and its cumulative effect on animal tissues. Sodium fluoride is described as being used in controlling the host of schistosomiasis (Biomphalaria glabrata). With a view to preserve the aquatic environment, it is proposed to verify the effect of sodium fluoride on fish (Poecilia vivipara). Ten fishes were divided into two groups. The first group was exposed to freshwater with sodium fluoride with a concentration of 5 ppm for a period of 21 days; the second group was exposed to freshwater. After 21 days the animals were sacrificed and their gills removed and processed according to histochemical/histologic methods and ultrastructural study. The gill leaflets were diaphanized in xylol. Histologic analyses using hematoxyline-eosin identified branchial epithelium and revealed mucous and chloride cells. The histochemical methods to detect glycoconjugate contents of mucous cells used: P.A.S, P.A.S. + salivar amylase; P.A.S. + acetylation ; P.A.S. + reversible acetylation; Alcian blue pH 2.5; Alcian blue pH 0.5; Alcian blue pH 2.5 + methylation; and Alcian blue pH 2.5 + reversible methylation. An increase in secretion of mucus and an alteration to the content of the granules were also observed, suggesting behavior changes of mucous type to enable these animals to adapt to the environment, thus altering the concentration of sodium fluoride.

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Published
2004-08-01
How to Cite
Breseghelo, L., Cardoso, M., Borges-de-Oliveira, R., Costa, M., Barreto, J., Sabóia-Morais, S., & Yamada, Áureo. (2004). Effects of sodium fluoride in gill epithelium of Guppy fish (Poecilia vivipara). Brazilian Journal of Veterinary Research and Animal Science, 41(4), 274-280. https://doi.org/10.1590/S1413-95962004000400009
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