Detection of Brucella spp. DNA in samples of blood and vaginal or preputial swab from dogs in the county of Natal, Rio Grande do Norte, Brazil

  • Annielle Regina da Fonsêca Fernandes Universidade Federal de Campina Grande, Unidade Acadêmica de Medicina Veterinária do Centro de Saúde e Tecnologia Rural, Patos, PB
  • Pomy de Cássia Peixoto Kim Universidade Federal Rural de Pernambuco, Departamento de Medicina Veterinária, Recife, PE
  • Sabrina Barros de Araújo Dantas Universidade Federal de Campina Grande, Unidade Acadêmica de Medicina Veterinária do Centro de Saúde e Tecnologia Rural, Patos, PB
  • Rinaldo Aparecido Mota Universidade Federal Rural de Pernambuco, Departamento de Medicina Veterinária, Recife, PE
  • Sérgio Santos Azevedo Universidade Federal de Campina Grande, Unidade Acadêmica de Medicina Veterinária do Centro de Saúde e Tecnologia Rural, Patos, PB
Keywords: Brucellosis, Dogs, PCR, Blood, Vaginal swab, Preputial swab

Abstract

The aim of this work was to detect Brucella spp. DNA in samples of blood and vaginal or preputial swabs in 80 seropositive dogs for brucellosis by agar gel immunodiffusion test (AGID) from the county of Natal, Rio Grande do Norte State, Brazil. Whole blood samples were collected with anticoagulant (sodium citrate) and vaginal and preputial swab samples for DNA extraction and polymerase chain reaction (PCR) employing ITS66 and ITS279 primers. Six animals showed amplification of Brucella spp., being one animal in both samples, two dogs only in blood samples, and three only in reproductive tract swabs. It is concluded that infection due to Brucella spp. occurs in dogs from the county of Natal, and the detection of DNA of the agent in reproductive tract swabs may be used as complementary tool in the diagnosis of canine brucellosis.

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Published
2013-06-21
How to Cite
Fernandes, A. R. da, Kim, P. de C., Dantas, S., Mota, R. A., & Azevedo, S. (2013). Detection of Brucella spp. DNA in samples of blood and vaginal or preputial swab from dogs in the county of Natal, Rio Grande do Norte, Brazil. Brazilian Journal of Veterinary Research and Animal Science, 50(3), 206-210. https://doi.org/10.11606/issn.1678-4456.v50i3p206-210
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