Leishmania sp. infection in dogs from Florianópolis, Santa Catarina, SC, Brazil

  • Acácio Duarte Pacheco Universidade Estadual Paulista, Faculdade de Medicina Veterinária, Departamento de Clínica, Cirurgia e Reprodução Animal, Araçatuba, SP
  • Marcia Dalastra Laurenti Universidade de São Paulo, Faculdade de Medicina, Departamento de Patologia, São Paulo, SP
  • Valéria Marçal Félix de Lima Universidade Estadual Paulista, Faculdade de Medicina Veterinária, Departamento de Clínica, Cirurgia e Reprodução Animal, Araçatuba, SP
  • Thaise Yumie Tomokane Universidade de São Paulo, Faculdade de Medicina, Departamento de Patologia, São Paulo, SP
  • Mary Marcondes Universidade Estadual Paulista, Faculdade de Medicina Veterinária, Departamento de Clínica, Cirurgia e Reprodução Animal, Araçatuba, SP
Keywords: Visceral Leishmaniasis, Diagnosis, ELISA, IFAT, PCR

Abstract

The aim of the present study was to investigate the occurrence of Leishmania sp. infection in dogs (N = 491) living in the municipality of Florianópolis, Santa Catarina (SC), Brazil, which was considered a disease-free region for visceral leishmaniasis until 2011, when autochthonous cases of canine disease were notified. Seroprevalence in this population was assessed by ELISA (0.4%; 2/491) and IFAT (4.09%; 24/491). Only one dog exhibited seroreactivity in both serological methods, comprising a total of 25 (5.3%) seroreagent animals. Leishmania sp. DNA, obtained from a sample of whole blood of this animal, was amplified by both conventional and Real-Time PCR. Sequencing of the amplified DNA and, thereby, determination of the Leishmania species involved, was not possible. Our results suggest the necessity of a thorough epidemiological investigation in Florianópolis.

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Published
2013-06-21
How to Cite
Pacheco, A., Laurenti, M., Lima, V., Tomokane, T., & Marcondes, M. (2013). Leishmania sp. infection in dogs from Florianópolis, Santa Catarina, SC, Brazil. Brazilian Journal of Veterinary Research and Animal Science, 50(3), 220-225. https://doi.org/10.11606/issn.1678-4456.v50i3p220-225
Section
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