Efficacy of anti-leptospirosis bacterines. Relation between the results of the in vitro growth inhibition test and the potency test, in vivo, with challenge in hamsters

  • Amane Paldês Gonçalves Universidade de Santo Amaro, São Paulo, SP
  • Gisele Oliveira de Souza Universidade de São Paulo, São Paulo, SP
  • Flávia Morato Universidade de São Paulo, São Paulo, SP
  • Zenaide Maria de Morais Universidade de São Paulo, São Paulo, SP
  • Sérgio Santos Azevedo Universidade Federal de Campina Grande, Campina Grande, PB
  • Silvio Arruda Vasconcellos Universidade de São Paulo, São Paulo, SP
Keywords: Animal leptospirosis, Hamsters, Vaccines, Potency text, In vitro growth inibition test and potency

Abstract

It was performed a comparison between the protection afforded in hamsters and the antibody levels induced by two commercial vaccines against leptospirosis. The assays used were the official challenge test (TP), the in vitro leptospires growth inhibition test (ICLIV) and microscopic agglutination test (MAT). The immunization protocol consisted of two single applications, 15 days from each other, of 0.25 mL of the bacterins, pure or its two-fold serial dilutions: 200 to 51,200 for bacterin A and 200 to 3.200 bacterin B, both of them administered subcutaneously. A group of animals was challenged, after 15 days from the second vaccine application, with 0.2 mL/animal of live leptospire cultures, with Canicola (bacterin A and B) or Kennewicki (bacterin A) serovars. The numbers of infective doses employed in the challenges were 100 and 631 for Canicola and Kennewicki serovars, respectively. After 15 days from the second vaccine dose the groups of animals used in ICLIV and SAM tests were bled and their sera were collected in pools (n = 5). In TP, adopting the criteria established by the Code Federal Regulation, both bacterins were approved. The comparison of the performance of the tested bacterins with the adopted serovars, according to its concentration, by the proportions of surviving animals to the challenge assay and the average of the neutralizing antibodies titers, established a neutralizing antibodies titer equal or higher than 0.77 log corresponding with the bacterin level of approval in the potency assay.

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Published
2013-10-29
How to Cite
Gonçalves, A., Souza, G., Morato, F., Morais, Z. M. de, Azevedo, S., & Vasconcellos, S. (2013). Efficacy of anti-leptospirosis bacterines. Relation between the results of the in vitro growth inhibition test and the potency test, in vivo, with challenge in hamsters. Brazilian Journal of Veterinary Research and Animal Science, 50(5), 370-378. https://doi.org/10.11606/issn.2318-3659.v50i5p370-378
Section
ARTICLES