Effect of transport temperature of ovaries on in vitro maturation of canine oocytes collected in different stages of the estrous cycle

  • Leda Maria Costa Pereira Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal e Radiologia Veterinária
  • Paulo Ricardo Oliveira Bersano Universidade Estadual do Ceara, Faculdade de Veterinária Universidade Estadual Paulista “Júlio de Mesquita Filho”, Centro Estudos em Venenos de Animais Peçonhentos
  • Maria Denise Lopes Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal e Radiologia Veterinária
Keywords: In vitro maturation, Temperature, Anestrus, Diestrus, Bitch

Abstract

This study evaluated the influence of estrous cycle stage and transport temperature of ovaries on in vitro maturation of canine oocytes. The bitches were categorized into two groups based on stage of estrus cycle: diestrus or anestrus. One ovary of each pair collected was transported in saline solution at 4°C while the other was transported at 37°C. Thus, ovarian tissue was sliced in PBS to release cumulus oocyte complexes (COCs). A total of 345 COCs (n = 186 oocytes from ovaries of bitches in anestrus and 159 in diestrus) were cultivated in TCM 199 supplemented with HEPES, sodium pyruvate, cysteine, follicle stimulating hormone (FSH), human chorionic gonadotropin (hCG), estrogen (E2) and epidermal growth factor (EGF). After 72h of maturation, the COCs were denuded, fixed and stained to assess nuclear maturation. The Fisher test was applied to examine the differences between the groups. The significance level adopted was 0.05. The oocytes obtained from the ovaries from bitches in diestrus transported at 4°C shown increased frequency of oocytes in metaphase II stage than those maintained at 37°C (p < 0.01). Similarly, there was increased frequency of oocytes in metaphase II (11.1%) stage from the ovaries of bitches in anestrus and transported at 4°C, than those maintained at 37°C (p < 0.05). It was concluded that transport temperature influences the results of canine oocyte viability and in vitro maturation, regardless of reproductive stage of the female.

Downloads

Download data is not yet available.

Author Biographies

Leda Maria Costa Pereira, Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal e Radiologia Veterinária
Doutoranda em Reprodução Animal, Departamento de Reprodução Animal e Radiologia Veterinária- Área Reprodução Animal- Laboratório de Pequenos animais e silvestres
Paulo Ricardo Oliveira Bersano, Universidade Estadual do Ceara, Faculdade de Veterinária Universidade Estadual Paulista “Júlio de Mesquita Filho”, Centro Estudos em Venenos de Animais Peçonhentos

Professor Adjunto de Patologia Veterinária, Faculdade de Veterinária, Universidade Estadual do Ceará, FAVET-UECE, Fortaleza, CE.

Pós-Doutorando pelo Centro Estudos em Venenos de Animais Peçonhentos, CEVAP-UNESP, Campus de Botucatu, Botucatu, SP.

Maria Denise Lopes, Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal e Radiologia Veterinária
Professora Titular, Departamento de Reprodução Animal e Radiologia Veterinária - UNESP, Campus de Botucatu, Botucatu, SP.
Published
2015-06-30
How to Cite
Pereira, L., Bersano, P., & Lopes, M. (2015). Effect of transport temperature of ovaries on in vitro maturation of canine oocytes collected in different stages of the estrous cycle. Brazilian Journal of Veterinary Research and Animal Science, 52(2), 158-166. https://doi.org/10.11606/issn.1678-4456.v52i2p158-166
Section
ARTICLES