Determining organochlorine pesticides in samples of green sea turtles by QuEChERS approach

  • Angélica María Sánchez-Sarmiento Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Patologia, Laboratório de Patologia Comparada de Animais Selvagens
  • Franz Zirena Vilca Universidade de São Paulo, Laboratório de Ecotoxicologia, Centro de Energia Nuclear na Agricultura
  • Silmara Rossi Universidade de São Paulo, Escola Superior de Agricultura Luiz de Queiroz, Centro de Energia Nuclear na Agricultura
  • Sergio Henrique Monteiro Instituto Biológico, Centro de Pesquisa e Desenvolvimento em Proteção Ambiental
  • Luiz Américo da Silva do Vale Universidade de São Paulo, Instituto de Química, Grupo de Pesquisa em Química Verde e Ambiental
  • Valdemar Luiz Tornisielo Universidade de São Paulo, Laboratório de Ecotoxicologia, Centro de Energia Nuclear na Agricultura
  • Eliana Reiko Matushima Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Patologia, Laboratório de Patologia Comparada de Animais Selvagens
Keywords: Chelonia mydas, Electron capture detector, Fat, Liver, Persistent Organic Pollutants (POPs)

Abstract

Some Organochlorine Pesticides (OCPs) can pose numerous adverse effects on biota. Marine turtles face numerous threats, in particular those related to anthropogenic activities. Therefore, development and improvement methodologies for monitoring chemical compounds are a relevant task. In this work, we developed a methodology based on the QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) extraction for detection of twelve OCPs, by gas chromatography with electron capture detector, in fat and liver samples of green sea turtles. Quantification limits were lower than 5.3 ng g-1; acceptable recovery rates for most compounds; medium matrix effect; matrix-calibration with linearity at the range from 1.0 to 200 ng g-1. This methodology provides contributions for the study of pesticide residues with adverse effects on sea turtle health, important skills for new directions in conservation issues. 

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Published
2016-04-12
How to Cite
Sánchez-Sarmiento, A. M., Vilca, F., Rossi, S., Monteiro, S. H., Vale, L., Tornisielo, V. L., & Matushima, E. (2016). Determining organochlorine pesticides in samples of green sea turtles by QuEChERS approach. Brazilian Journal of Veterinary Research and Animal Science, 53(1), 97-102. https://doi.org/10.11606/issn.1678-4456.v53i1p97-102
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