Research of Salmonella and sanitary conditions in poultry and sausages retailed in Botucatu

Authors

  • Vera Lúcia Mores Rall Universidade Estadual Paulista, Departamento de Microbiologia e Imunologia, Botucatu, SP
  • José Guilherme Prado Martin Universidade Estadual Paulista, Departamento de Microbiologia e Imunologia, Botucatu, SP
  • João Manuel Grisi Candeias Universidade Estadual Paulista, Departamento de Microbiologia e Imunologia, Botucatu, SP
  • Karen Franco Godoy Cardoso Universidade Estadual Paulista, Departamento de Microbiologia e Imunologia, Botucatu, SP
  • Márcia Guimarães da Silva Universidade Estadual Paulista, Faculdade de Medicina, Departamento de Patologia, Botucatu, SP
  • Ricardo Rall Faculdade de Tecnologia, Botucatu, SP
  • João Pessoa Araújo Júnior Universidade Estadual Paulista, Departamento de Microbiologia e Imunologia, Botucatu, SP

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2009.26763

Keywords:

Poultry, Sausage, Coliforms, Salmonella, PCR

Abstract

In the present investigation were evaluated the sanitary conditions of poultry and several types of sausages retailed in Botucatu, São Paulo, Brazil for the determination of the most probable number of coliforms at 45ºC/g besides the research of Salmonella using traditional methodology and PCR. In order to do so, 50 samples of poultry and 75 of sausages were collected from nine different establishments in the city, in the period of April to November of 2006. Of the 50 samples of chicken meat, 35 (70%) were out of the microbiologic parameters, according to Brazilian Sanitary Resolution RDC nº12 of Anvisa (>;10(4) coliforms at 45ºC/g). In this Resolution, the research of Salmonella is not demanded, but 4 samples (8%) presented the pathogen using the traditional methodology. That presence was confirmed by PCR, which was also positive in another 23, in a total of 27 positive samples (54%). Among 75 samples of sausages, 30 (40%) were out of the allowed limits, with 7 positive samples for Salmonella, using traditional methodology. However, if we consider PCR test, the number of positive samples increases to 42 (56%). Adding this number to coliforms microbiological limits, 86.7% of the analyzed sausages were inappropriate for the consumption.

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Published

2009-06-01

Issue

Section

UNDEFINIED

How to Cite

Research of Salmonella and sanitary conditions in poultry and sausages retailed in Botucatu. (2009). Brazilian Journal of Veterinary Research and Animal Science, 46(3), 167-174. https://doi.org/10.11606/issn.1678-4456.bjvras.2009.26763