Canine distemper virus: detection of viral RNA by Nested RT-PCR in dogs with clinical diagnosis

Authors

  • Rosana Alcalde Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP
  • Marcia Mery Kogika Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, SP
  • Vera A. Batistini Fortunato Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, SP
  • Luciano Rodrigo Lopes Universidade Federal de São Paulo, Escola Paulista de Medicina, Departamento de Informática em Saúde, São Paulo, SP
  • Paulo Bandeira Paiva Universidade Federal de São Paulo, Escola Paulista de Medicina, Departamento de Informática em Saúde, São Paulo, SP
  • Edison Luiz Durigon Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP

DOI:

https://doi.org/10.11606/issn.2318-3659.v50i1p74-76

Keywords:

Distemper, Nested-PCR, CDV

Abstract

Canine distemper virus (CDV) is a pathogen which affects dogs and causes severe disease leading to death. Dogs infected with CDV can be diagnosed by RNA detection by Nested PCR technique. The following study proposed to valuate CDV RNA in blood, urine and saliva samples. The Nested-PCR technique was able to detect CDV RNA in different types of biologic samples. The higher number of positive results was obtained in urine samples.

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Published

2013-02-22

Issue

Vol. 50 No. 1 (2013)

Section

PRELIMINARY NOTE

License

The journal content is authorized under the Creative Commons  BY-NC-SA  license (summary of the license: https://creativecommons.org/licenses/by-nc-sa/4.0 | full text of the license: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode) 

How to Cite

1.
Alcalde R, Kogika MM, Fortunato VAB, Lopes LR, Paiva PB, Durigon EL. Canine distemper virus: detection of viral RNA by Nested RT-PCR in dogs with clinical diagnosis. Braz. J. Vet. Res. Anim. Sci. [Internet]. 2013 Feb. 22 [cited 2024 Apr. 19];50(1):74-6. Available from: https://revistas.usp.br/bjvras/article/view/55844