Prophylactic application of laser light restores L-FABP expression in the livers of rats submitted to partial ischemia

Authors

  • Kelvin Henrique Vilalva Universidade de Sao Paulo. Faculdade de Medicina de Ribeirao Preto. Departamento de Cirurgia e Anatomia
  • Rebeca Lopes Figueira Universidade de Sao Paulo. Faculdade de Medicina de Ribeirao Preto. Departamento de Cirurgia e Anatomia
  • Marina Silveira Universidade de Sao Paulo. Faculdade de Medicina de Ribeirao Preto. Departamento de Cirurgia e Anatomia
  • Catarina Graf Universidade de Sao Paulo. Faculdade de Medicina de Ribeirao Preto. Departamento de Cirurgia e Anatomia
  • Frances Lanhellas Gonçalves Universidade de Sao Paulo. Faculdade de Medicina de Ribeirao Preto. Departamento de Cirurgia e Anatomia
  • Lourenço Sbragia Universidade de Sao Paulo. Faculdade de Medicina de Ribeirao Preto. Departamento de Cirurgia e Anatomia
  • Maria Cecília Gomes Universidade de Sao Paulo. Faculdade de Medicina de Ribeirao Preto. Departamento de Cirurgia e Anatomia
  • Fabricía Mumic Universidade de Sao Paulo. Faculdade de Medicina de Ribeirao Preto. Departamento de Cirurgia e Anatomia
  • Jose Dirceu Vollet-Filho Universidade de Sao Paulo. Instituto de Fisica de Sao Carlos. Departamento de Fisica e Ciencia dos Materiais
  • Vanderlei Salvador Bagnato Universidade de Sao Paulo. Instituto de Fisica de Sao Carlos. Departamento de Fisica e Ciencia dos Materiais
  • Luiz Augusto Carneiro D’Albuquerque Universidade de Sao Paulo. Faculdade de Medicina. Departamento de Gastroenterologia
  • Orlando Castro-e-Silva Universidade de Sao Paulo. Faculdade de Medicina de Ribeirao Preto. Departamento de Cirurgia e Anatomia

DOI:

https://doi.org/10.6061/clinics/2018/e113

Keywords:

Ischemia, Reperfusion Injury, Laser Therapy, L-FABP

Abstract

OBJECTIVES: The objective of the present study was to evaluate the protective effect of pre-conditioning treatment with laser light on hepatic injury in rats submitted to partial ischemia using mitochondrial function and liver fatty acid binding protein as markers. METHODS: Rats were divided into four groups (n=5): 1) Control, 2) Control + Laser, 3) Partial Ischemia and 4) Partial Ischemia + Laser. Ischemia was induced by clamping the hepatic pedicle of the left and middle lobes of the liver for 60 minutes. Laser light at 660 nm was applied to the liver immediately prior to the induction of ischemia at 22.5 J/cm2 , with 30 seconds of illumination at five individual points. The animals were sacrificed after 30 minutes of reperfusion. Blood and liver tissues were collected for analysis of mitochondrial function, determination of malondialdehyde and analysis of fatty acid binding protein expression by Western blot. RESULTS: Mitochondrial function decreased in the Partial Ischemia group, especially during adenosine diphosphate-activated respiration (state 3), and the expression of fatty acid binding protein was also reduced. The application of laser light prevented bioenergetic changes and restored the expression of fatty acid binding protein. CONCLUSION: Prophylactic application of laser light to the livers of rats submitted to partial ischemia was found to have a protective effect in the liver, with normalization of both mitochondrial function and fatty acid binding protein tissue expression.

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Published

2019-02-13

Issue

Section

Original Articles

How to Cite

Prophylactic application of laser light restores L-FABP expression in the livers of rats submitted to partial ischemia. (2019). Clinics, 73, e113. https://doi.org/10.6061/clinics/2018/e113