Screening of Strongyloides infection using an ELISA test in transplant candidates

Authors

  • Beatriz Toledo Universidade de Sao Paulo, Faculdade de Medicina, Hospital das Clinicas, Laboratorio de Investigacao Medica (LIM/06 – Laboratorio de Imunopatologia da esquistossomose)
  • Marcelo A. Corral Universidade de Sao Paulo, Faculdade de Medicina, Hospital das Clinicas, Laboratorio de Investigacao Medica (LIM/06 – Laboratorio de Imunopatologia da esquistossomose)
  • Dirce Mary C. L. Meisel Universidade de Sao Paulo, Faculdade de Medicina, Hospital das Clinicas, Laboratorio de Investigacao Medica (LIM/06 – Laboratorio de Imunopatologia da esquistossomose)
  • Maiara Gottardi Universidade de Sao Paulo, Faculdade de Medicina, Hospital das Clinicas, Laboratorio de Investigacao Medica (LIM/06 – Laboratorio de Imunopatologia da esquistossomose)
  • Edson Abdala Universidade de Sao Paulo, Faculdade de Medicina, Hospital das Clinicas https://orcid.org/0000-0003-0765-6654
  • Silvia F. Costa Universidade de Sao Paulo, Faculdade de Medicina, Hospital das Clinicas
  • Lígia Camera Pierrotti Universidade de Sao Paulo, Faculdade de Medicina, Hospital das Clinicas https://orcid.org/0000-0002-0216-3028
  • Susana A. Z. Lescano Universidade de Sao Paulo, Faculdade de Medicina, Hospital das Clinicas, Laboratorio de Investigacao Medica (LIM/06 – Laboratorio de Imunopatologia da esquistossomose)
  • Elenice M. N. Gonc¸alves Universidade de Sao Paulo, Faculdade de Medicina, Hospital das Clinicas, Secao de Parasitologia da Divisao de Laboratorio Central
  • Vera L. P. Castilho Universidade de Sao Paulo, Faculdade de Medicina, Hospital das Clinicas, Secao de Parasitologia da Divisao de Laboratorio Central
  • Pedro P. Chieff V Faculdade de Ciencias Medicas, Santa Casa
  • Ronaldo C. B. Gryschek Universidade de Sao Paulo, Faculdade de Medicina, Hospital das Clinicas, Laboratorio de Investigacao Medica (LIM/06 – Laboratorio de Imunopatologia da esquistossomose)
  • Fabiana M. Paula Universidade de Sao Paulo, Faculdade de Medicina, Hospital das Clinicas, Laboratorio de Investigacao Medica (LIM/06 – Laboratorio de Imunopatologia da esquistossomose) https://orcid.org/0000-0003-3343-0149

DOI:

https://doi.org/10.6061/clinics/2019/e698

Keywords:

Heterologous Antigen, Strongyloides Venezuelensis, ELISA Test, Transplant Candidates

Abstract

OBJECTIVES: Hyperinfection or disseminated strongyloidiasis has been frequently reported after transplants and is related to high mortality. This study aimed to screen for strongyloidiasis using serological diagnoses in transplant candidates. METHODS: An ELISA test was performed with filariform larvae of Strongyloides venezuelensis as a source of antigen. RESULTS: In the serum from transplant candidates, anti-Strongyloides IgG antibodies were detected in 35/150 (23.3%) samples by soluble fractions in phosphate buffered saline (PBS), 31/150 (20.7%) samples by soluble fractions in Tris-HCl, 27/150 (18.0%) samples by membrane fractions in PBS and 22/150 (14.7%) samples by membrane fractions in Tris-HCl. CONCLUSIONS: The present results suggest the ELISA test, ideally using soluble fractions of filariform larvae S. venezuelensis in PBS, as an additional strategy for the diagnosis of strongyloidiasis in transplant candidates.

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Published

2019-06-12

Issue

Vol. 74 (2019)

Section

Original Articles

How to Cite

Screening of Strongyloides infection using an ELISA test in transplant candidates. (2019). Clinics, 74, e698. https://doi.org/10.6061/clinics/2019/e698