Comparison of the biocompatibility of grey mineral trioxide aggregate and sealapex plus zinc oxide in rat subcutaneous tissue
Objectives:The aim of this study was to compare the subcutaneous tissue response to grey mineral trioxide aggregate white Sealapex plus zinc oxide. Methods: Polyethylene tubes filled with tested material were implanted in the connective tissue of rats. Control animals received empty tubes. Tissue samples were collected after 7, 60, and 90 days and stained with hematoxylin-eosin, picrosirius-fast green, and von Kossa stain for morphological analysis. The connective tissue response to the implanted materials was evaluated descriptively and semi-quantitatively by scoring the degree of inflammation, granulation tissue formation, fibrosis, and calcification. Results: Examinations of the grey mineral trioxide aggregate group over time revealed more intense inflammation at 7 days than at 60 days (p <0.05). In the Sealapex plus zinc oxide group, granulation tissue was more abundant at 7 days than at 60 days (p <0.05). Regarding calcification, von Kossa-positive granules were observed in the grey mineral trioxide aggregate and Sealapex plus zinc oxide groups at all time points studied. In the Sealapex/ZnO group, calcification was more apparent at 60 days than at 7 days (p <0.05). Relevance: This study demonstrates that all tested materials promote similar tissue reactions.
Descriptors: Biocompatibility Testing, Endodontics, Dental Materials, Retrograde Obturation.
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