Adhesion evaluation of Candida albicans on the surface of termopolymerizable acrylic resins treated with photopolymerizable coatings and/or silica nanoparticles addition
an in vitro study
Keywords:Oral Candidiasis, Acrylic Resins, Cell Adhesion, Silica
To evaluate how the addition of 0.5% silica nanoparticles and/or photopolymerizable coatings affect the adhesion of Candida albicans on the surface of acrylic resin PMMA (poly-methyl methacrylate). Materials and methods: A total of 40 specimens were fabricated (65 mm × 10 mm × 2,5 mm) and randomly divided into four experimental groups according to surface treatment using silica and/or photopolymerizable coating: the control group (G1); silica group (G2) (0.5% silica); photopolymerizable coating group (G3); and silica group (0.5% silica) + photopolymerizable coatings (G4). Specimens were separately incubated with a Candida albicans suspension spectrophotometrically standardized with 1 × 107 CFU/ml at 37 °C for 90 minutes. After this period, the specimens were removed and then their fungal adhesion was analyzed by the colonyforming units counting. Data were analyzed by the One-way ANOVA test followed by the Tukey test (P ≤ 0,05). Results: A significant reduction in Candida albicans adhesion was observed in G2, G3, and G4 when compared to the control group G1 (P ≤ 0.0001). However, similar fungal adhesion was observed when G2, G3, and G4 were compared (P ≤ 0.05). Conclusions: The addition of silica nanoparticles and/or photopolymerizable coating on the surface of PMMA decreases the adhesion of Candida albicans, which can be used as a strategy to reduce oral lesions caused by acrylic dentures.
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