Adhesion evaluation of Candida albicans on the surface of termopolymerizable acrylic resins treated with photopolymerizable coatings and/or silica nanoparticles addition

an in vitro study

Authors

  • Helidior Teixeira Lopes Centro Universitário da Serra Gaúcha
  • Liliane Alves Centro Universitário da Serra Gaúcha
  • Alexandra Flávia Gazzoni Centro Universitário da Serra Gaúcha

DOI:

https://doi.org/10.11606/issn.2357-8041.clrd.2019.157790

Keywords:

Oral Candidiasis, Acrylic Resins, Cell Adhesion, Silica

Abstract

To evaluate how the addition of 0.5% silica nanoparticles and/or photopolymerizable coatings affect the adhesion of Candida albicans on the surface of acrylic resin PMMA (poly-methyl methacrylate). Materials and methods: A total of 40 specimens were fabricated (65 mm × 10 mm × 2,5 mm) and randomly divided into four experimental groups according to surface treatment using silica and/or photopolymerizable coating: the control group (G1); silica group (G2) (0.5% silica); photopolymerizable coating group (G3); and silica group (0.5% silica) + photopolymerizable coatings (G4). Specimens were separately incubated with a Candida albicans suspension spectrophotometrically standardized with 1 × 107 CFU/ml at 37 °C for 90 minutes. After this period, the specimens were removed and then their fungal adhesion was analyzed by the colonyforming units counting. Data were analyzed by the One-way ANOVA test followed by the Tukey test (P ≤ 0,05). Results: A significant reduction in Candida albicans adhesion was observed in G2, G3, and G4 when compared to the control group G1 (P ≤ 0.0001). However, similar fungal adhesion was observed when G2, G3, and G4 were compared (P ≤ 0.05). Conclusions: The addition of silica nanoparticles and/or photopolymerizable coating on the surface of PMMA decreases the adhesion of Candida albicans, which can be used as a strategy to reduce oral lesions caused by acrylic dentures.

Downloads

Download data is not yet available.

Author Biographies

Helidior Teixeira Lopes, Centro Universitário da Serra Gaúcha

Departamento de Odontologia, Centro Universitário da Serra Gaúcha (FSG), Caxias do Sul, RS, Brasil.

Liliane Alves, Centro Universitário da Serra Gaúcha

Departamento de Odontologia, Centro Universitário da Serra Gaúcha (FSG), Caxias do Sul, RS, Brasil.

Alexandra Flávia Gazzoni, Centro Universitário da Serra Gaúcha

Laboratório de Patologia e Microbiologia Oral, Centro Universitário da Serra Gaúcha (FSG), Caxias do Sul, RS, Brasil.

References

Pan H, Wang G, Pan J, Ye G, Sun K, Zhang J, et al. Cold plasma-induced surface modification of heat-polymerized acrylic resin and prevention of early adherence of Candida albicans. Dent Mater J. 2015;34(4):529-36. doi:10.4012/dmj.2015-035.

Cevik P, Yildirim-Bicer AZ. The effect of silica and prepolymer nanoparticles on the mechanical properties of denture base acrylic resin. J Prosthodont. 2018;27(8):763-70. doi: 10.1111/jopr.12573.

da Silva LH, Feitosa SA, Valera MC, de Araújo MA, Tango RN. Effect of the addition of silanated silica on the mechanical properties of microwave heat-cured acrylic resin. Gerodontology. 2012;29(2):e1019-23. doi: 10.1111/j.1741-2358.2011.00604.x.

Vanden Abbeele A, de Meel H, Ahariz M, Perraudin JP, Beyer I, Courtois P. Denture contamination by yeasts in the elderly. Gerodontology. 2008;25(4):222-8. doi: 10.1111/j.1741-2358.2007.00247.x.

Koch C, Bürgers R, Hahnel S. Candida albicans adherence and proliferation on the surface of denture base materials. Gerodontology. 2013;30(4):309-13. doi: 10.1111/ger.12056.

Mayahara M, Kataoka R, Arimoto T, Tamaki Y, Yamaguchi N, Watanabe Y, et al. Effects of surface roughness and dimorphism on the adhesion of Candida albicans to the surface of resins: scanning electron microscope analyses of mode and number of adhesions. J Investig Clin Dent. 2014;5(4):307-12. doi: 10.1111/jicd.12055.

Susewind S, Lang R, Hahnel S. Biofilm formation and Candida albicans morphology on the surface of denture base materials. Mycoses. 2015;58(12):719-27. doi: 10.1111/myc.12420.

Gendreau L, Loewy ZG. Epidemiology and etiology of denture stomatitis. J Prosthodont. 2011;20(4):251-60. doi: 10.1111/j.1532-849X.2011.00698.x.

Hamid SK, Al-Dubayan AH, Al-Awami H, Khan SQ, Gad MM. In vitro assessment of the antifungal effects of neem powder added to polymethyl methacrylate denture base material. J Clin Exp Dent. 2019;11(2):e170-e178. doi: 10.4317/jced.55458.

Qian K, Pan H, Li Y, Wang G, Zhang J, Pan J. Time-related surface modification of denture base acrylic resin treated by atmospheric pressure cold plasma. Dent Mater J. 2016;35(1):97-103. doi: 10.4012/dmj.2015-162.

Hong RY, Fu HP, Zhang YJ, Liu L, Wang J, Li HZ, et al. Surface-modified silica nanoparticles for reinforcement of PMMA. J Appl Polym Sci. 2007; 105(4):2176-84. doi: 10.1002/app.26164.

Sivakumar I, Arunachalam KS, Sajjan S, Ramaraju AV, Rao B, Kamaraj B. Incorporation of antimicrobial macromolecules in acrylic denture base resins: a research composition and update. J Prosthodont. 2014;23(4):248-90. doi: 10.1111/jopr.12105.

Stieven E, Conde A, Galafassi D, Elsemann EB, Elsemann RB, Gazzoni AF. Análise da utilização de nanopartículas de prata e/ou plasma de baixa pressão e temperatura para a prevenção de Candida albicans em próteses dentárias. R-BITS. 2018; 8(1):67-7.

Qasim M, Singh BR, Nagvi AH, Paik P, Das D. Silver nanoparticles embedded mesoporous SiO2 nanosphere: an effective anticandidal agent against Candida albicans 077. Nanotechnology. 2015;26(28):285102. doi: 10.1088/0957-4484/26/28/285102.

Lee JH, El-Fiqi A, Jo JK, Kim DA, Kim SC, Jun SK, et al. Development of long-term antimicrobial poly(methyl methacrylate) by incorporating mesoporous silica nanocarriers. Dent Mater. 2016;32(12):1564-74. doi: 1016/j.dental.2016.09.001.

Yodmongkol S, Chantarachindawong R, Thaweboon S, Thaweboon B, Amornsakchai T, Srikhirin T. The effects of silane-SiO2 nanocomposite films on Candida albicans adhesion and the surface and physical properties of acrylic resin denture base material. J Prosthet Dent. 2014;112(6):1530-8. doi:10.1016/j.prosdent.2014.06.019.

Cousins BG, Allison HE, Doherty PJ, Edwards C, Garvey MJ, Martin DS, et al. Effects of a nanoparticulate silica substrate on cell attachment of Candida albicans. J Appl Microbiol. 2007;102(3):757-65. doi: 10.1111/j.1365-2672.2006.03124.x.

Monsenego P. Presence of microorganisms on the fitting denture complete surface: study “in vivo”. J Oral Rehabil. 2000:27(8);708-13. doi: 10.1046/j.1365-2842.2000.00564.x.

Yano J, Yu A, Fidel PL Jr, Noverr MC. Candida glabrata has no enhancing role in the pathogenesis of Candida-associated denture stomatitis in a rat model. mSphere. 2019;4(2):pii:e00191-19. doi: 10.1128/mSphere.00191-19.

Banting DW, Hill SA. Microwave disinfection of dentures for the treatment of oral candidiasis. Spec Care Dentist. 2001;21(1):4-8.

Cağavi F, Akalan N, Celik H, Gür D, Güçiz B. Effect of hydrophilic coating on microorganism colonization in silicone tubing. Acta Neurochir (Wien). 2004;146(6):603-10. doi: 10.1007/s00701-004-0262-z.

Lazarin AA, Zamperini CA, Vergani CE, Wady AF, Giampaolo ET, Machado AL. Candida albicans adherence to an acrylic resin modified by experimental photopolymerised coatings: an in vitro study. Gerodontology. 2014;31(1):25-33. doi: 10.111/j.1741-2358.2012.00688.x.

Lazarin AA, Machado AL, Zamperini CA, Wady AF, Spolidorio DM, Vergani CE. Effect of experimental photopolymerized coatings on the hydrophobicity of a denture base acrylic resin and on Candida albicans adhesion. Arch Oral Biol. 2013;58(1):1-9. doi: 10.1016/j.archoralbio.212.10.005.

Revised American Dental Association specification nº 12 for denture base polymers. J Am Dent Assoc. 1975;90(2):39-49. doi: 10.14219/jada.archive.1975.0069.

Ramage G, Tomsett K, Wicks BL, López-Ribot JL, Redding SW. Denture stomatitis: a role for Candida biofilms. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004;98(1):53-9. doi: 10.1016/j.tripleo.2003.04.002.

Curtis A, Wilkinson C. Nantotechniques and approaches in biotechnology. Trends Biotechnol. 2001;19(3):97-101.

Hirota K, Murakami K, Nemoto K, Miyake Y. Coating of a surface with 2-methacryloyloxyethyl phosphorylcholine (MPC) co-polymer significantly reduces retention of human pathogenic microorganisms. FEMS Microbiol Lett. 2005;248(1):37-45. doi: 10.1016/j.femsle.2005.05.019.

Zissis AJ, Polyzois GL, Yannikakis SA, Harrison A. Roughness of denture materials: a comparative study. Int J Prosthodont. 2000;13(2):136-40.

Published

2019-12-06

Issue

Section

Original Research