Adhesion evaluation of Candida albicans on the surface of termopolymerizable acrylic resins treated with photopolymerizable coatings and/or silica nanoparticles addition

an in vitro study

  • Helidior Teixeira Lopes Centro Universitário da Serra Gaúcha
  • Liliane Alves Centro Universitário da Serra Gaúcha
  • Alexandra Flávia Gazzoni Centro Universitário da Serra Gaúcha
Keywords: Oral Candidiasis, Acrylic Resins, Cell Adhesion, Silica


To evaluate how the addition of 0.5% silica nanoparticles and/or photopolymerizable coatings affect the adhesion of Candida albicans on the surface of acrylic resin PMMA (poly-methyl methacrylate). Materials and methods: A total of 40 specimens were fabricated (65 mm × 10 mm × 2,5 mm) and randomly divided into four experimental groups according to surface treatment using silica and/or photopolymerizable coating: the control group (G1); silica group (G2) (0.5% silica); photopolymerizable coating group (G3); and silica group (0.5% silica) + photopolymerizable coatings (G4). Specimens were separately incubated with a Candida albicans suspension spectrophotometrically standardized with 1 × 107 CFU/ml at 37 °C for 90 minutes. After this period, the specimens were removed and then their fungal adhesion was analyzed by the colonyforming units counting. Data were analyzed by the One-way ANOVA test followed by the Tukey test (P ≤ 0,05). Results: A significant reduction in Candida albicans adhesion was observed in G2, G3, and G4 when compared to the control group G1 (P ≤ 0.0001). However, similar fungal adhesion was observed when G2, G3, and G4 were compared (P ≤ 0.05). Conclusions: The addition of silica nanoparticles and/or photopolymerizable coating on the surface of PMMA decreases the adhesion of Candida albicans, which can be used as a strategy to reduce oral lesions caused by acrylic dentures.


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Author Biographies

Helidior Teixeira Lopes, Centro Universitário da Serra Gaúcha

Departamento de Odontologia, Centro Universitário da Serra Gaúcha (FSG), Caxias do Sul, RS, Brasil.

Liliane Alves, Centro Universitário da Serra Gaúcha

Departamento de Odontologia, Centro Universitário da Serra Gaúcha (FSG), Caxias do Sul, RS, Brasil.

Alexandra Flávia Gazzoni, Centro Universitário da Serra Gaúcha

Laboratório de Patologia e Microbiologia Oral, Centro Universitário da Serra Gaúcha (FSG), Caxias do Sul, RS, Brasil.


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Original Research