Effect of laser therapy on IGF-1 in the parotid and submandibular glands of streptozotocin-induced diabetic rats

Authors

  • Danielle Lima Corrêa de Carvalho Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo, São Paulo, SP
  • José Nicolau Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo, São Paulo, SP
  • Alyne Simões Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo, São Paulo, SP

DOI:

https://doi.org/10.11606/issn.2357-8041.clrd.2014.59344

Keywords:

Diabetes Mellitus, Blood Glucose, Insulin-Like Growth Factor I, Laser Therapy, Salivary Glands.

Abstract

Diabetes mellitus is a metabolic disease of multiple etiologies that leads to hyperglycemia and can cause numerous dysfunctions and failure of organs and tissues, including the salivary glands. Some studies using diabetic rats have shown a decrease in glucose blood concentration when low-power laser (LPL) was used on salivary glands; however, the mechanism of action of lasers on carbohydrate metabolism is yet unknown. Thus, the aim of this study was to assess whether LPL irradiation on salivary glands can change the IGF-1 concentration of diabetic rats. Thirty-eight female rats were divided into 4 groups: D0 and D5 (diabetic animals) and C0 and C5 (control animals), resrespectively irradiated with 0 and 5 J/cm2. Diabetes was induced by administration of streptozotocin and confi rmed later by the glycaemia results. Twenty-nine days after induction, the parotid and submandibular glands of groups D5 and C5 were irradiated with a diode laser. Twenty-four hours after irradiation, the animals were sacrifi ced and their salivary glands were collected to assess IGF-1 concenconcentration. Diabetic animals that received irradiation showed lower glucose concentration on the day of sacrifi ce in comparison with the day they had been diagnosed (p ≤ 0.05); however, IGF-1 concentration was unchanged by irradiation. Based on the results of this study, it was concluded that LPL irradiation can decrease blood glucose concentration of diabetic animals; however, this effect appears to be unrelated to the concentration of IGF-1.

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Author Biographies

  • Danielle Lima Corrêa de Carvalho, Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo, São Paulo, SP
    Departamento de Biomateriais e Biologia Oral e Área Biologia Oral.
  • José Nicolau, Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo, São Paulo, SP
    Departamento de Biomateriais e Biologia Oral e Área Biologia Oral.
  • Alyne Simões, Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo, São Paulo, SP
    Departamento de Biomateriais e Biologia Oral e Área Biologia Oral.

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Published

2014-09-08

Issue

Vol. 20 No. 3 (2014)

Section

Original Research

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