Expression of PCNA, p53 and Ki-67 in dentinogenic ghost cell tumors and calcifying cystic odontogenic tumors

  • Bianca de Fátima Borim Pulino Department of Stomatology, School of Dentistry, University of São Paulo, São Paulo
  • Carla Silva Siqueira Department of Stomatology, School of Dentistry, University of São Paulo, São Paulo
  • Carina Magalhães Esteves Duarte Department of Stomatology, School of Dentistry, University of São Paulo, São Paulo
  • Suzana Cantanhede Orsini Machado de Sousa Department of Stomatology, School of Dentistry, University of São Paulo
Keywords: Odontogenic Cyst, Calcifying, Cell Proliferation, Immunohistochemistry.

Abstract

The aim of this study was to compare the proliferative pattern of dentinogenic ghost cell tumors (DGCT) and unicystic calcifying cystic odontogenic tumors (UCCOT) using antibodies against PCNA, p53 and Ki- 67. Three cases diagnosed as DGCT and ten as UCCOT were retrieved from the fi les of the Surgical Oral Pathology Service at the University of São Paulo, Brazil. Immunohistochemistry was performed in formalin- fi xed and paraffi n-embedded tissue specimens. Only nuclear staining was considered specifi c for the three antibodies. Immunoexpression of PCNA, Ki-67 and p53 was analyzed semi-quantitatively and quantitatively. The results showed that PCNA was positive in both lesions, mainly in the basal cells, and p53 showed positivity in all cases. UCCOT showed statistically signifi cantly higher Ki-67 labeling indices than did DGCT. In conclusion, our results do not support the correlation between proliferative activity as shown by these proteins and reported biologic behavior.

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Published
2014-02-26
Section
Original Research