Efeitos do laser GaAs e alongamento na contusão muscular em ratos
DOI:
https://doi.org/10.1590/1809-2950/13903823012016Resumo
Laser e alongamento são usados para tratar lesões musculares. Este estudo objetivou avaliar os efeitos do laser GaAs e alongamento na morfologia do músculo Tibial anterior (TA) após contusão. Trinta e seis ratos (349±23 g) foram divididos em seis grupos (n=6): grupo controle (GC); grupo lesão e laser (GLL); grupo lesão e alongamento (GLA); grupo lesão, laser e alongamento (GLLA) e grupo alongamento (GA). Foi realizada lesão no TA por meio de um aparato de contusão. O tratamento com laser GAAS foi usado com dose de 4,5 J/cm2 durante 32 s, iniciando 48 h após lesão, por 7 dias. Alongamento passivo manual consistiu de 10 repetições de 1 minuto de duração, iniciando no 8º dia, 3 vezes por semana, durante 3 semanas. Após 4 semanas, os ratos foram eutanasiados para retirada do TA para análise de: peso e comprimento musculares, área de secção transversa das fibras musculares (ASTFM), número de sarcômeros em série (NSS), comprimento dos sarcômeros e porcentagem de tecido conjuntivo. A comparação entre os grupos deu-se por meio da ANOVA e post hoc Tukey, com nível de significância ≤ 0,05. O número de sarcômeros em série do GLLS foi maior que o GLS. O comprimento dos sarcômeros no GLA foi superior ao GLL, GLLA e GA. No GA houve aumento do NSS comparado com o GC, enquanto a porcentagem de tecido conjuntivo do GA diminuiu em comparação com o GLLA. Assim, a sarcomerogênese dos músculos lesionados foi aumentada pelo uso do laser, alongamento e pela associação destes. O alongamento foi suficiente para aumentar o NSS em músculos intactos.Downloads
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