Effect of γ-lactones and γ-lactams compounds on Streptococcus mutans biofilms

  • Mariane Beatriz Sordi Universidade Federal de Santa Catarina; Departamento de Odontologia; Centro de Ensino e Pesquisa em Implantes Dentários
  • Thaís Altoé Moreira Universidade Federal de Minas Gerais; Instituto de Ciências Exatas; Departamento de Química
  • Juan Felipe Dumes Montero Universidade Federal de Santa Catarina; Departamento de Odontologia; Centro de Ensino e Pesquisa em Implantes Dentários
  • Luis Cláudio Barbosa Universidade Federal de Minas Gerais; Instituto de Ciências Exatas; Departamento de Química
  • César Augusto Magalhães Benfatti Universidade Federal de Santa Catarina; Departamento de Odontologia; Centro de Ensino e Pesquisa em Implantes Dentários
  • Ricardo de Souza Magini Universidade Federal de Santa Catarina; Departamento de Odontologia; Centro de Ensino e Pesquisa em Implantes Dentários
  • Andréa de Lima Pimenta Université de Cergy-Pontoise; Département de Biologie
  • Júlio César Matias de Souza Universidade Federal de Santa Catarina; Departamento de Odontologia; Centro de Ensino e Pesquisa em Implantes Dentários
Keywords: Lactones, Lactams, Biofilm, Antimicrobial, Streptococcus mutans

Abstract

Considering oral diseases, antibiofilm compounds can decrease the accumulation of pathogenic species such as Streptococcus mutans at micro-areas of teeth, dental restorations or implant-supported prostheses. Objective To assess the effect of thirteen different novel lactam-based compounds on the inhibition of S. mutans biofilm formation. Material and methods We synthesized compounds based on γ-lactones analogues from rubrolides by a mucochloric acid process and converted them into their corresponding γ-hydroxy-γ-lactams by a reaction with isobutylamine and propylamine. Compounds concentrations ranging from 0.17 up to 87.5 μg mL-1 were tested against S. mutans. We diluted the exponential cultures in TSB and incubated them (37°C) in the presence of different γ-lactones or γ-lactams dilutions. Afterwards, we measured the planktonic growth by optical density at 630 nm and therefore assessed the biofilm density by the crystal violet staining method. Results Twelve compounds were active against biofilm formation, showing no effect on bacterial viability. Only one compound was inactive against both planktonic and biofilm growth. The highest biofilm inhibition (inhibition rate above 60%) was obtained for two compounds while three other compounds revealed an inhibition rate above 40%. Conclusions Twelve of the thirteen compounds revealed effective inhibition of S. mutans biofilm formation, with eight of them showing a specific antibiofilm effect.

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Published
2018-01-01
Section
Original Articles