Quality of cellular attachment to various root-end filling materials

Authors

  • Ahmad S. AL-Hiyasat Jordan University of Science and Technology; Faculty of Dentistry; Department of Conservative Dentistry
  • Oula R. Al-Sa'Eed Jordan University of Science and Technology; Faculty of Dentistry; Department of Conservative Dentistry
  • Homa Darmani Jordan University of Science and Technology; Faculty of Science; Department of Biotechnology and Genetic Engineering

DOI:

https://doi.org/10.1590/S1678-77572012000100015

Keywords:

Biocompatibility testing, Cell adhesion, Electron microscopy, Endodontics, Dental materials, Fibroblasts

Abstract

OBJECTIVES: This study investigated cellular attachment to 6 root-end filling materials as a measure of the biocompatibility of the materials. MATERIAL AND METHODS: Class I retrograde cavities were prepared in root slices and these cavities were filled with the test materials, and incubated with Balb/C 3T3 fibroblasts for 24 h. Root slices with the cavities left empty served as the controls. The root slices were then processed for scanning electron microscopy, and were viewed to assess the quality of cellular attachment by observing the shape of cells, spread, and membrane outline. RESULTS: The best cellular attachment was observed at MTA and Geristore surfaces: cells exhibited characteristic elongated fibroblastic morphology, with projections of lamellipodia, filopodia, blebs, and microvilli from their surfaces, reflecting good attachment to the material. Fibroblasts attached poorly to the surfaces of IRM, Super EBA, KetacFil and Retroplast. Furthermore, the cells did not attach well to the tooth structure next to IRM and Super EBA. CONCLUSIONS: The present study demonstrated a variation in cellular attachment to different root-end filling materials with the best cellular attachment to the surfaces of MTA and Geristore. IRM and Super EBA, Ketac Fil and Retroplast rendered poor attachment.

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Published

2012-02-01

Issue

Section

Original Articles

How to Cite

Quality of cellular attachment to various root-end filling materials . (2012). Journal of Applied Oral Science, 20(1), 82-88. https://doi.org/10.1590/S1678-77572012000100015