Gross brain morphology of Rhamdia quelen ( Quoy & Gaimard 1824 ) ( Ostariophysi : Siluriformes : Heptapteridae )

The brain gross morphology of Rhamdia quelen is described and compared with seven species of six genera of Heptapteridae. Interspecific variation in shape, size, and position of brain subdivisions was observed in all examined species. The posterior position of the hypophysis on the hypothalamus and presence of a lateral subdivision on the lobus facialis are shared by all examined heptapterids. Rhamdia quelen and Pimelodella gracilis, currently considered closely related within the family Heptapteridae, exhibit the anterior and posterior area of the telencephalon with equivalent widths, and the lateral line lobe reaching the anterior area of the lobus vagi. Members of the so called Nemuroglanis sub-clade (Cetopsorhamdia iheringi, Heptapterus mustelinus, Imparfinis mirini, and Phenacorhamdia tenebrosa) share the lobus vagi proportional smaller than the lobus facialis; the lateral line lobe reaching the half length of the lobus facialis; the tectum mesencephali in contact with the telencephalon, and thinner anterior area of the telencephalon. The results reveal several features that are phylogenetically informative among the heptapterids examined, and corroborate previous hypotheses based on other non-neural anatomical characters. Key-Words. Central nervous system; Anatomy; Catfish; Neotropical region.

Among Siluriformes, Heptapteridae currently includes 218 valid species of small to me-dium-sized catfishes distributed in 24 genera (Eschmeyer & Fong, 2018).They live among rocks and logs on river bottoms, associated to marginal vegetation or buried in sand (Bockmann & Guazelli, 2003).The family was first proposed a monophyletic group within the Pimelodidae by Lundberg & McDade (1986), and later corroborated by Ferraris Jr. (1988) and Lundberg et al. (1991), in which members of the Heptapteridae were included in the subfamily Rhamdiinae.Recent studies regarding the phylogenetic relationships on catfishes, based on molecular analysis, have proposed that the Heptapteridae is more related to a clade composed by the Pimelodidae, Pseudopimelodidae and Conorhynchos (Hardman, 2005;Sullivan et al., 2006;Sullivan et al., 2013).Bockmann (1998), on the other hand, was the first to propose a more encompassing phylogenetic relationship of the Heptapteridae, recognizing some synapomorphies, based on the external morphology and osteology, for several species groups.
In the Heptapteridae, the genus Rhamdia Bleeker 1858 can be diagnosed by a unique combination of nine characters (Silfvergrip, 1996), and nowadays includes 27 species (Eschmeyer & Fong, 2018).Rhamdia quelen (Quoy & Gaimard, 1824) is widely distributed from northern Mexico to southern South America, in both cis-and trans-Andean watersheds, and is one of the most interesting taxa in the genus due to sympatric and even syntopic occurrence with other congeners.Although the ubiquitous distribution throughout the neotropical region, the complicated taxonomic history and the vast list of synonymies, the species can be distinguished by a set of 12 character states (Silfvergrip, 1996).However, there is disagreement as to the validity of these characters that can embrace several different morphotypes under a single name (Angrizani & Malabarba, 2018).
Few studies on morphological diversity within the Heptapteridae have been published to date (e.g., Lundberg & McDade, 1986;Silfvergrip, 1996;Bockmann & Miquelarena, 2008;Slobodian & Bockmann, 2013), and the literature is even scarce when concerning the brain gross morphology of this group.Herein, the brain gross morphology and major cranial nerves of the R. quelen are described.Anatomical including seven other representatives of the family Heptapteridae are also made.Finally, intrafamiliar characters evolution is discussed.

MATERIAL AND METHODS
Brain gross morphology was obtained from six dissected specimens of R. quelen  and compared to seven other species of the Heptapteridae.Dissections were performed following Abrahão & Pupo (2014) and Abrahão & Shibatta (2015).A digital camera attached to a stereoscopic microscope was used to capture images of the brain topography.Measurements of the major brain topographic units were taken on digital images, using the software Axio Vision Rel v.4.8 (Carl Zeiss Axio Vision Product Suite), and are expressed and presented as proportions of the total brain length (BL), see Table 1.Total brain length was measured from the rostral portion of the telencephalon to the most caudal portion of the lobus vagi (modified from Lannoo & Eastman, 2000).Only the lobus inferior hypothalami was measured in the diencephalon, because this region has visible topography and with well-defined boundaries.Neuroanatomy nomenclature follows Meek & Nieuwenhuys (1998) and Table 1.Morphometric data of major subdivisions of the brain of members of the Heptapteridae.Total length of brain (BL) in millimeters, other characters in proportions of BL.SD = Standard deviation; n = number of specimens.Butler & Hodos (2005).Osteological nomenclature follows Silfvergrip (1996), Bockmann (1998), andArratia et al. (2003).Brains were kept in 4% formalin buffered with CaCO₃, after removal from the neurocranium.All specimens were preserved in 70% ethanol after fixation in 4% formalin.The software R (R Core Team, 2017) was used to perform the statistical analysis.

RESULTS
Measurements of the major brain subdivisions of the species examined are presented in Table 1.The brain is completely located beneath the supraoccipital and frontal bones, and above the parasphenoid and prootic in species of Heptapteridae, with exception of the bulbus olfactorius and part of the anterior portion of the telencephalon (Fig. 1).

Rhombencephalon
In all species examined the medulla oblongata is located posterior to the efferent projections of the nervus vagus to the medulla spinalis and is positioned dorsal to the truncus cerebri.The anterior portion lies posterolateral to the lobus vagi, and is positioned above the parasphenoid-basioccipital suture, beneath the supraoccipital process (Fig. 1).The medulla oblongata is topographically composed of two ovoid-shaped structures contacting the medulla spinalis.The posterior portion of the medulla oblongata is slightly thinner than their counterparts (Fig. 2).In Rhamdia quelen (Fig. 2), Imparfinis mirini, and Phenacorhamdia tenebrosa (Fig. 3), the medulla oblongata does not possess a conspicuous bulge posterolateral to the lobus vagi.In Goeldiella eques, Cetopsorhamdia iheringi, Heptapterus mustelinus and Pimelodella gracilis, the medulla oblongata is slightly bulged posterolaterally (Fig. 3).The length of the medulla oblongata in relation to the BL is similar in R. quelen, G. eques and I. mirini, although significantly different from the other examined species (Table 1).
The lobus vagi is located at the dorsal portion of the rhombencephalon, positioned beneath the supraoccipital and supraoccipital process (Fig. 1).In dorsal view, these lobes lie immediately posterior and continuous to the lobus facialis and are anteromedially located to the medulla oblongata.In all species examined the lobus vagi is composed of two cylindrical, paired, V-shaped lobes, with anterior bulges.These lobes contact each other only in their posterior portions, forming an acute tip (Figs. 2 and 3).The lobus vagi is longer than the lobus facialis in R. quelen, G. eques and P. gracilis.In all remaining species examined the inverse was found.The lobus vagi length in relation to BL is substantially smaller in H. mustelinus (Fig. 3, Table 1).The presence of a conspicuous bulge on the anterior portion of lobus vagi is shared by all Heptapteridae species examined herein (Fig. 3).
The lobus facialis is located at the dorsal portion of the rhombencephalon, positioned beneath the supraoccipital process (Fig. 1).In dorsal view, it is posterior to the corpus cerebelli, anterior to the lobus vagi, and medially located in relation to the lateral line lobe.A small portion of the anterior area of the lobus facialis is positioned beneath the posterior area of the corpus cerebelli (Fig. 2).The lobes of the lobus facialis are bilaterally symmetrical and do not contact each other.In all species examined these lobes are longitudinally elongate, somewhat rectangular-shaped, but with rounded edges in dorsal view.The lobus facialis is continuous with the lobus vagi and the lateral line lobe, but not with the corpus cerebelli.There is a conspicuous bulge laterally positioned over to each lobe of the lobus facialis (Figs. 2 and 3).Except for H. mustelinus and P. gracilis, the proportional length of the lobus facialis is larger than the length of the lateral line lobe in all species examined (Table 1).The length of the lobus  1).
The lateral line lobe is located at the dorsal portion of the rhombencephalon and is positioned beneath the supraoccipital (Fig. 1).It lies posterolateral to the eminentia granularis and the corpus cerebelli and is located laterally in relation to the lobus facialis.The lateral line lobe is formed by two conspicuous bulges, ovoid-shaped, with two subdivisions each one.The anterior bulge is located posterolaterally to the eminentia granularis and the corpus cerebelli, while the posterior bulge is positioned lateral to the lobus facialis.In all examined species the anterior bulge is more prominent than the posterior bulge (Figs. 2, 3 and 4).In R. quelen, G. eques and P. gracilis the lateral line lobe extends up to the anterior portion of the lobus vagi, whereas in all remaining species these lobes extend up to the half-length of the lobus facialis (Figs. 2 and 3).In all examined species, the proportional width of the lateral line lobe is larger than to the medulla oblongata and smaller than the length of the lobus vagi (Table 1).The width of the lateral line lobe relative to BL is similar in R. quelen and G. eques.The width of this lobe is larger in P. gracilis, and smaller in H. mustelinus and I. mirini (Table 1).The eminentia granularis is located at the dorsal region of the rhombencephalon, positioned beneath the supraoccipital bone (Fig. 1).In dorsal and lateral views, it is posterolateral to the posterior area of the corpus cerebelli, anterior to the lateral line lobe, and posterior to the tectum mesencephali.The lobes of the eminentia granularis are somewhat cylindrical in dorsal view, and ovalshapped with the posterior portion slightly smaller than the anterior one, in lateral view.The corpus cerebelli is located at the dorsal portion of the rhombencephalon, positioned beneath the supraoccipital (Fig. 1).This lobe is located immediately anterior to the lobus facialis, dorsal to the hypothalamus, and medial to the lobes of the tectum mesencephali.The anterior portion of this structure is dorsal to the posterior area of the telencephalon.The corpus cerebelli has a trapezoid shape, with the anterior region slightly smaller than the posterior one.The dorsal margin in lateral view and the lateral margin in dorsal view are straight, without conspicuous undulations (Fig. 2).In R. quelen and P. gracilis the anterior and posterior margins in dorsal view are also straight, whereas in G. eques the anterior portion is straight, and the posterior portion has two flaps (Fig. 3).In H. mustelinus, I. mirini, and C. iheringi the anterior portion of the corpus cerebelli is rounded, whereas the caudal portion is flap-shaped (Fig. 3).In P. tenebrosa the anterior portion is pointed and the posterior portion is flap-shaped (Fig. 3).The corpus cerebelli contacts the telencephalon in all examined species (Fig. 4).In all examined species the corpus cerebelli is the largest subdivision of the brain.The proportional length of the corpus cerebelli in relation to BL is longer in H. mustelinus, intermediate in R. quelen and P. gracilis, and shorter in G. eques and I. mirini (Table 1).
The efferent projections of the nervus glossopharyngeus and the nervus vagus emerge from the lateral wall of the rhombencephalon, approximately at half-length of the lobus vagi.These branches pass through the braincase by a foramen in the exoccipital.The nervus octavus, and nervus linea lateralis posterior emerge from the ventrolateral face of the rhombencephalon, at approximately the mid-point of the lateral line lobe.The nervus facialis and nervus trigeminus emerge from the ventrolateral wall  of the rhombencephalon, anterior to the nervus octavus, exiting the efferent projections of the braincase through a foramen on the suture between the parasphenoid, prootic and pterosphenoid.The anterior branches of the nervus facialis and nervus trigeminus exit their efferent projections from the braincase through a single foramen between the pterosphenoid, frontal and orbitosphenoid (Fig. 1).

Mesencephalon
The tectum mesencephali is located at the dorsal portion of the tegmentum mesencephalic, lateral to the corpus cerebelli in dorsal view and posterior to the telencephalon in lateral view.The lobes of the tectum mesencephali are positioned beneath the supraoccipital and frontal bone (Fig. 1).This subdivision has two bilateral, almost spherical, lobes in dorsal and lateral views (Fig. 2).In R. quelen, G. eques and P. gracilis these lobes contact the corpus cerebelli and the telencephalon.In all remaining representatives, the tectum mesencephali contact only the corpus cerebelli (Fig. 3).In all specimens, the length of the tectum mesencephali is proportional shorter than the length of the corpus cerebelli, telencephalon, and the lobus inferior hypothalami (Table 1).The length of the tectum mesencephali in relation to BL is substantially shorter in I. mirini (Table 1).
The nervus opticus (nII) emerges from the tectum mesencephali on the mesencephalon, and its efferent projections exit in the region immediately anterior to the lobus inferior hypothalamic.These fibers contact each other and cross the midline of the brain at the region of the chiasma mesencephalic.The efferent projections of the nervus opticus exit the braincase through a foramen located between the frontal and orbitosphenoid, near the pterosphenoid (Fig. 1).

Diencephalon
The lobus inferior hypothalami is located at the ventral portion of the diencephalon, in a posterior region to the chiasma mesencephali, ventral to the truncus cerebri and the tectum mesencephalic, lateral to the hypothalamus in ventral view, and posterior to the telencephalon in lateral view (Fig. 2).The lobes of the lobus inferior hypothalami are positioned above the parasphenoid and prootic (Fig. 1).The lobus inferior hypothalami is semicircular, with the anterior portion slightly smaller than the posterior portion.The hypophysis remains anchored posteriorly on the lobus hypothalamus and is located between the lobes of the lobus inferior hypothalami (Fig. 2).The hypothalamus is also located at the ventral portion of the diencephalon, medially and above the lobus inferior hypothalamic.The lobes of the hypothalamus are rounded.The proportional length of the lobus inferior hypothalami is shorter than the telencephalon only in R. quelen, G. eques and H. mustelinus (Table 1).The length of this subdivision in relation to BL is longer in H. mustelinus (Table 1).

Telencephalon
In all examined specimens, the telencephalon is located anterior to the tectum mesencephali in lateral view, posterior to the bulbus olfactorius, and with the posterior area positioned beneath the corpus cerebelli in dorsal and lateral views (Fig. 2).The lobes of the telencephalon are positioned completely beneath the frontal (Fig. 1).In I. mirini the telencephalon does not contact the corpus cerebelli (Fig. 3).The telencephalon is longitudinally elon-gate, like a cylinder, with both the anterior and posterior margins rounded.In all examined species the anterior margin of the telencephalon is slightly smaller than the posterior one.In R. quelen and P. gracilis the anterior and posterior margins of these lobes have equivalent widths.In all remaining examined species the anterior portion is slight smaller than the posterior one (Fig. 3).There are no substantial differences in the proportional length of this subdivision among species examined (Table 1).
The bulbus olfactorius is stalked and is positioned at the anterior portion of the brain.This structure is located beneath the nasal, near to the articulation between the lateral ethmoid and the vomer (Fig. 1).The bulbus olfactorius is rounded, with equivalent widths of its anterior and posterior margins.It is connected to the olfactory epithelium via the nervus olfactorius, and to the telencephalon via the tractus olfactorius (Fig. 2).Length and shape variations of the bulbus olfactorius were not found.The olfactory epithelium is rounded, with the anterior margin slight smaller than the posterior one.It has a considerable amount of lamellae on each side, granting it a feather appearance (Fig. 2).

DISCUSSION
Morphometric and gross morphology variations were found on the brains of the examined species.No minor intraspecific variation and sexual dimorphism in brains were observed.These combinations of remarkable intergeneric and limited intraspecific variations suggest that characters derived from brain anatomy may be useful for phylogenetic inferences.
The brain gross morphology of R. quelen and other species of the Heptapteridae examined here share some features with other members of the Pimelodoidea, a clade corroborated by morphological (Lundberg et al., 1991;Bockmann, 1998;Bockmann & Guazzelli 2003;Lundberg & Littmann, 2003;Shibatta, 2003;Birindelli & Shibatta, 2011) and molecular-based data (Hardman, 2005;Sullivan et al., 2006;Sullivan et al., 2013).These shared conditions and characters are: (1) the lobus vagi composed of two cylindrical, paired, and V-shaped lobes, and (2) the lateral line lobe composed of two conspicuous bulges, ovoid-shaped, with the anterior portion more prominent than the posterior one.Contrasting to Pimelodidae and Pseudopimelodidae, members of the Heptapteridae share the following putative synapomorphies: (1) the position of the hypophysis at the posterior area of the hypothalamus (vs.anchored at the midpoint of the hypothalamus) and (2) the presence of lateral subdivision on the lobus facialis (vs.anterolateral positioned).
According to brain gross morphology of R. quelen and other examined species of Heptapteridae, a spectrum of variation is notable where at one end are located the genera Rhamdia, Pimelodella and Goeldiella, and on the other all the remaining heptapterids examined herein.While the first three taxa exhibit the telencephalon with a tumid anterior area, along the relative smaller length of the lobus facialis, all other Heptapteridae taxa have Abrahão, V.P. et al.: Brain of Rhamdia quelen Pap. Avulsos Zool., 2018;v.58: e20185842 8/10 the telencephalon with thinner anterior area, along the relative longer length of the lobus facialis.The proportional length of the lobus vagi longer than the lobus facialis, the lateral line lobe extending up to the anterior portion of the lobus vagi, and the tectum mesencephali contacting the corpus cerebelli and the general shape of the telencephalon are features present in R. quelen, P. gracilis and G. eques.The widths of the anterior and posterior portions of the telencephalon and their proportional length, and the straight posterior portion of the corpus cerebelli are complementary features present in R. quelen and P. gracilis (Fig. 3 and Table 1).Based on morphological and osteological features, Bockmann (1998) proposed that the genus Goeldiella was sister group of all other heptapterids, however there is no consensus on Rhamdia and Pimelodella position.The phylogenetic relationship at the base of Heptapteridae cladogram remain uncertain (Bockmann & Miquelarena, 2008;Slobodian & Bockmann, 2013).Future comprehensive research on Pimelodoidea neuroanatomy may contribute to elucidate those assumptions.
The length of the lobus facialis proportionally greater than length of the lobus vagi, the lateral line lobe extending up to the half-length of the lobus facialis, the tectum mesencephali not contacting the telencephalon, and the shape of the posterior and anterior portions of the telencephalon, are all traits found in Cetopsorhamdia iheringi, Heptapterus mustelinus, Imparfinis mirini, and Phenacorhamdia tenebrosa (Fig. 3; Table 1).This separation agrees with the proposal of Ferraris Jr. (1988) and Bockmann (1994) who included Cetopsorhamdia, Heptapterus, Imparfinis and Phenacorhamdia, in the Nemuroglanis sub-clade, as a distinct group within heptapterids.
The shape, position, and proportional lengths of the brain as a whole, and its different subdivisions, corroborate the relationship hypothesis using external morphology and osteological characters presented by Ferraris Jr. (1988) and Bockmann (1998).These brain characters may allow recognition and resolution of smaller lineages within the family if incorporated to a broader phylogenetic reconstruction of the group.
Abrahão, V.P. et al.: Brain of Rhamdia quelen facialis in relation to BL is longer in R. quelen, G. eques and I. mirini, and shorter in H. mustelinus (Table