Vancomycin monitoring in term newborns: comparison of peak and trough serum concentrations determined by high performance liquid chromatography and fluorescence polarization immunoassay

Authors

  • Rubens Feferbaum University of São Paulo; Faculty of Medicine; Hospital das Clínicas; Department of Pediatrics
  • José Kleber Kobol Machado University of São Paulo; Faculty of Medicine; Hospital das Clínicas; Department of Pediatrics
  • Edna Maria de Albuquerque Diniz University of São Paulo; Faculty of Medicine; Hospital das Clínicas; Department of Pediatrics
  • Thelma S. Okay University of São Paulo; Faculty of Medicine; Hospital das Clínicas; Department of Pediatrics
  • Silvia R. C. J. Santos University of São Paulo; Faculty of Medicine; Hospital das Clínicas; Department of Pediatrics
  • Maria Esther J.R. Ceccon University of São Paulo; Faculty of Medicine; Hospital das Clínicas; Department of Pediatrics
  • Vera L. J. Krebs University of São Paulo; Faculty of Medicine; Hospital das Clínicas; Department of Pediatrics
  • Maria C. K. de Araújo University of São Paulo; Faculty of Medicine; Hospital das Clínicas; Department of Pediatrics
  • Flávio Adolfo Costa Vaz University of São Paulo; Faculty of Medicine; Hospital das Clínicas; Department of Pediatrics

DOI:

https://doi.org/10.1590/S0041-87812001000500004

Keywords:

Chromatography, high pressure liquid, Fluorescence polarization immunoassay, Vancomycin, Sepsis, Newborn infant

Abstract

INTRODUCTION: Peak and trough serum concentrations of vancomycin were determined in term newborn infants with confirmed or suspected Staphylococcus sp sepsis by high performance liquid chromatography and flourescence polarization immunoassay. OBJECTIVE: To statistically compare the results of the high performance liquid chromatography and flourescence polarization immunoassay techniques for measuring serum vancomycin concentrations. METHODS: Eighteen peak and 20 trough serum samples were assayed for vancomycin concentrations using high performance liquid chromatography and flourescence polarization immunoassay from October 1995 to October 1997. RESULTS: The linear correlation coefficients for high performance liquid chromatography and flourescence polarization immunoassay were 0.27 (peak, P = 0.110) and 0.26 (trough, P = 0.1045) respectively, which were not statistically significant. CONCLUSION: There was wide variation in serum vancomycin concentrations determined by high performance liquid chromatography as compared with those determined by flourescence polarization immunoassay. There was no recognizable pattern in the variability; in an apparently random fashion, the high performance liquid chromatography measurement was sometimes substantially higher than the flourescence polarization immunoassay measurement, and at other times it was substantially lower.

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Published

2001-10-01

How to Cite

Feferbaum, R., Machado, J. K. K., Diniz, E. M. de A., Okay, T. S., Santos, S. R. C. J., Ceccon, M. E. J., Krebs, V. L. J., Araújo, M. C. K. de, & Vaz, F. A. C. (2001). Vancomycin monitoring in term newborns: comparison of peak and trough serum concentrations determined by high performance liquid chromatography and fluorescence polarization immunoassay . Revista Do Hospital Das Clínicas, 56(5), 149-152. https://doi.org/10.1590/S0041-87812001000500004

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Section

Original Articles