Study of Aedes aegypti population with emphasis on the gonotrophic cycle length and identification of arboviruses

implications for vector management in cemeteries

Authors

  • Julian E. Garcia-Rejon Universidad Autonoma de Yucatan
  • Armando Ulloa-Garcia Instituto Nacional de Salud Publica
  • Nohemi Cigarroa-Toledo Universidad Autonoma de Yucatan
  • Angelica Pech-May Instituto Nacional de Medicina Tropical
  • Carlos Machain-Williams Universidad Autonoma de Yucatan
  • Rosa Carmina Cetina-Trejo Universidad Autonoma de Yucatan
  • Lourdes Gabriela Talavera-Aguilar Universidad Autonoma de Yucatan
  • Oswaldo Margarito Torres-Chable Universidad Juarez Autonoma de Tabasco
  • Juan Carlos Navarro Universidad Internacional SEK, Facultad de Ciencias Naturales y Ambientales
  • Carlos Marcial Baak-Baak Universidad Autonoma de Yucatan

Keywords:

Arbovirus, Dengue, Mexico, Daily survival, Zika virus

Abstract

Aedes aegypti is the vector of the arboviruses causing dengue, chikungunya and zika infections in Mexico. However, its presence in public places has not been fully evaluated. In a cemetery from Merida, Yucatan, Mexico, the productivity of Ae. aegypti, the gonotrophic cycle, and the presence of Ae. aegypti females infected with arboviruses were evaluated. Immature and adult mosquitoes were inspected every two months between April 2016 to June 2017. For the gonotrophic cycle length, the daily pattern of total and parous female ratio was registered and was analyzed using time series analysis. Ae. aegypti females were sorted into pools and assayed for flavivirus RNA by RT-PCR and Sanger sequencing. Aedes aegypti immatures represented 82.86% (8,627/10,411) of the collection. In total, 1,648 Ae. aegypti females were sorted into 166 pools. Two pools were positive; one for dengue virus (DENV-1) and the other for zika virus (ZIKV). The phylogenetic analysis revealed that the DENV-1 is more closely related to isolates from Brazil. While ZIKV is more closely related to the Asian lineage, which were isolates from Guatemala and Mexico. We report some evidence of vertical transmission of DENV-1 in nulliparous females of Ae. aegypti. The gonotrophic cycle was four and three days in the rainy and dry season, respectively. The cemetery of Merida is an important focus of Ae. aegypti proliferation, and these environments may play a role in arboviruses transmission; probably limiting the efficacy of attempts to suppress the presence of mosquitoes in domestic environments.

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Published

2018-11-09

Issue

Section

Original Articles

How to Cite

Garcia-Rejon, J. E., Ulloa-Garcia, A., Cigarroa-Toledo, N., Pech-May, A., Machain-Williams, C., Cetina-Trejo, R. C., Talavera-Aguilar, L. G., Torres-Chable, O. M., Navarro, J. C., & Baak-Baak, C. M. (2018). Study of Aedes aegypti population with emphasis on the gonotrophic cycle length and identification of arboviruses: implications for vector management in cemeteries. Revista Do Instituto De Medicina Tropical De São Paulo, 60, e44. https://revistas.usp.br/rimtsp/article/view/151662