Cytomegalovirus in colorectal cancer and idiopathic ulcerative colitis

  • Viviane Casagrande Mariguela University of São Paulo; School of Medicine; Virology Research Center
  • Silvana Gama Florêncio Chacha University of São Paulo; School of Medicine; Department of Internal Medicine
  • Aldo de Albuquerque Cunha University of São Paulo; School of Medicine; Virology Research Center
  • Luiz Ernesto de Almeida Troncon University of São Paulo; School of Medicine; Department of Internal Medicine
  • Sérgio Zucoloto University of São Paulo; School of Medicine; Department of Pathology
  • Luiz Tadeu Moraes Figueiredo University of São Paulo; School of Medicine; Virology Research Center
Keywords: Cytomegalovirus, Colorectal cancer, Ulcerative colitis, Polymerase chain reaction, Immunohistochemistry

Abstract

Cytomegalovirus (CMV) is a genus in the family Herpesviridae that has been associated with gastrointestinal syndromes. In this work we looked for a possible association of CMV infection with colorectal cancer and ulcerative colitis (UC). Blood and enteric tissue samples of 14 patients with colorectal cancer and of 21 with UC were subjected to a nested-PCR that amplifies part of the gB gene of CMV and also to immunohistochemistry using a specific monoclonal antibody to IE 76kDa protein of CMV. CMV was detected by nested-PCR in the blood and/or the enteric tissue of nine (64.3%) colorectal cancer and 16 (76.2%) ulcerative colitis patients. In the immunohistochemistry it was observed that 12 (12/21, 57.1%) positive enteric tissue samples of patients with UC and none from patients with colorectal cancer (0/14) were positive to CMV. The positivity of CMV infections in the UC patient group (12/21, 57.1%) showed by both techniques, was significantly higher (p = 0.015) than that observed for colorectal cancer patients (2/14, 14.3%). These results suggest an association of ulcerative colitis with CMV infection of the enteric tissue.

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Published
2008-04-01
How to Cite
Mariguela, V., Chacha, S., Cunha, A., Troncon, L., Zucoloto, S., & Figueiredo, L. (2008). Cytomegalovirus in colorectal cancer and idiopathic ulcerative colitis . Revista Do Instituto De Medicina Tropical De São Paulo, 50(2), 83-87. Retrieved from http://www.revistas.usp.br/rimtsp/article/view/31157
Section
Virology