Comparison of five methods for oxacillin susceptibility testing of Staphylococcus aureus isolates from cystic fibrosis patients

Authors

  • Marcelo J. Mimica Santa Casa School of Medicine; Department of Pediatrics; Division of Infectious Diseases
  • Rozane L. Carvalho Santa Casa School of Medicine; Department of Pathology; Division of Microbiology
  • Eitan N. Berezin Santa Casa School of Medicine; Department of Pediatrics; Division of Infectious Diseases
  • Neiva Damaceno Santa Casa School of Medicine; Department of Pediatrics; Division of Pulmonology
  • Hélio H. Caiaffa-Filho FMUSP; Hospital das Clinicas; Laboratory of Medical Investigation

Keywords:

Oxacillin, Cefoxitin, Staphylococcus aureus, mecA, Cystic fibrosis, MRSA

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) are now a worldwide problem. Cystic fibrosis (CF) patients are commonly colonized and infected by MRSA. Accurate oxacillin susceptibility testing is mandatory for the adequate management of these patients. We performed a comparison of the accuracy of different tests in CF isolates, including methicillin-susceptible S. aureus and MRSA with different SCCmec types, and using the mecA gene as the gold-standard. The sensitivity and specificity of oxacillin disc, Etest, and oxacillin agar screening plate were 100%. Sensitivity of the cefoxitin disc was 85% and specificity was 100%. For clinically relevant isolates, laboratories may consider the use of a combination of two phenotypic methods.

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Published

2012-12-01

Issue

Section

Microbiology

How to Cite

Comparison of five methods for oxacillin susceptibility testing of Staphylococcus aureus isolates from cystic fibrosis patients. (2012). Revista Do Instituto De Medicina Tropical De São Paulo, 54(6), 305-306. https://revistas.usp.br/rimtsp/article/view/48421