HYPOXIC STRESS, HEPATOCYTES AND CACO-2 VIABILITY AND SUSCEPTIBILITY TO Shigella flexneri INVASION

Authors

  • Camila Barbara Cantalupo Lima University of Sao Paulo; Faculty of Medicine; Laboratory of Bacteriology (LIM 54); Department of Infectious Diseases
  • Sania Alves dos Santos University of Sao Paulo; Faculty of Medicine; Laboratory of Bacteriology (LIM 54); Department of Infectious Diseases
  • Dahir Ramos de Andrade Junior University of Sao Paulo; Faculty of Medicine; Laboratory of Bacteriology (LIM 54); Department of Infectious Diseases

Abstract

SUMMARY Inflammation due to Shigella flexneri can cause damage to the colonic mucosa and cell death by necrosis and apoptosis. This bacteria can reach the bloodstream in this way, and the liver through portal veins. Hypoxia is a condition present in many human diseases, and it may induce bacterial translocation from intestinal lumen. We studied the ability of S. flexneri to invade rat hepatocytes and Caco-2 cells both in normoxic and hypoxic microenvironments, as well as morphological and physiological alterations in these cells after infection under hypoxia. We used the primary culture of rat hepatocytes as a model of study. We analyzed the following parameters in normoxic and hypoxic conditions: morphology, cell viability, bacterial recovery and lactate dehydrogenase (LDH) released. The results showed that there were fewer bacteria within the Caco-2 cells than in hepatocytes in normoxic and hypoxic conditions. We observed that the higher the multiplicity of infection (MOI) the greater the bacterial recovery in hepatocytes. The hypoxic condition decreased the bacterial recovery in hepatocytes. The cytotoxicity evaluated by LDH released by cells was significantly higher in cells submitted to hypoxia than normoxia. Caco-2 cells in normoxia released 63% more LDH than hepatocytes. LDH increased 164% when hepatocytes were submitted to hypoxia and just 21% when Caco-2 cells were in the same condition. The apoptosis evaluated by Tunel was significantly higher in cells submitted to hypoxia than normoxia. When comparing hypoxic cells, we obtained more apoptotic hepatocytes than apoptotic Caco-2 cells. Concluding our results contribute to a better knowledge of interactions between studied cells and Shigella flexneri. These data may be useful in the future to define strategies to combat this virulent pathogen.

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Published

2013-09-01

How to Cite

Lima, C. B. C., Santos, S. A. dos, & Andrade Junior, D. R. de. (2013). HYPOXIC STRESS, HEPATOCYTES AND CACO-2 VIABILITY AND SUSCEPTIBILITY TO Shigella flexneri INVASION . Revista Do Instituto De Medicina Tropical De São Paulo, 55(5), 341-346. Retrieved from http://www.revistas.usp.br/rimtsp/article/view/78668

Issue

Section

Microbiology