SEROLOGICAL SURVEY OF Ehrlichia SPECIES IN DOGS, HORSES AND HUMANS: ZOONOTIC SCENERY IN A RURAL SETTLEMENT FROM SOUTHERN BRAZIL

  • Rafael Felipe da Costa Vieira Departmento de Ciências Veterinárias, Centro de Ciências Agrárias, Universidade Federal da Paraíba; Departmento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina
  • Thállitha Samih Wischral Jayme Vieira Departmento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina
  • Denise do Amaral Gomes Nascimento Departmento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina
  • Thiago F. Martins Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina, Veterinária e Zootecnia (FMVZ), Universidade de São Paulo
  • Felipe S. Krawczak Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina, Veterinária e Zootecnia (FMVZ), Universidade de São Paulo
  • Marcelo B. Labruna Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina, Veterinária e Zootecnia (FMVZ), Universidade de São Paulo
  • Ramaswamy Chandrashekar IDEXX Laboratories Inc
  • Mary Marcondes Departmento de Clínica, Cirurgia e Reprodução Animal, Universidade Estadual Paulista
  • Alexander Welker Biondo Departamento de Medicina Veterinária, Universidade Federal do Paraná; Department of Veterinary Pathobiology, University of Illinois
  • Odilon Vidotto Departmento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina
Keywords: Ehrlichia canis, Ehrlichia chaffeensis, IFA, ELISA.

Abstract

The aims of this study were to determine the seroprevalence of Ehrlichia spp. and risk factors for exposure in a restricted population of dogs, horses, and humans highly exposed to tick bites in a Brazilian rural settlement using a commercial ELISA rapid test and two indirect immunofluorescent assays (IFA) with E. canis and E. chaffeensis crude antigens. Serum samples from 132 dogs, 16 horses and 100 humans were used. Fifty-six out of 132 (42.4%) dogs were seropositive for E. canis. Dogs > one year were more likely to be seropositive for E. canis than dogs ≤ one year (p = 0.0051). Ten/16 (62.5%) and 8/16 (50%) horses were seropositive by the commercial ELISA and IFA, respectively. Five out of 100 (5%) humans were seropositive for E. canis and E. chaffeensis. Rhipicephalus sanguineus (n = 291, 97.98%) on dogs and Amblyomma cajennense (n = 25, 96.15%) on horses were the most common ticks found. In conclusion, anti-Ehrlichia spp. antibodies were found in horses; however, the lack of a molecular characterization precludes any conclusion regarding the agent involved. Additionally, the higher seroprevalence of E. canis in dogs and the evidence of anti-Ehrlichia spp. antibodies in humans suggest that human cases of ehrlichiosis in Brazil might be caused by E. canis, or other closely related species.

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Author Biography

Rafael Felipe da Costa Vieira, Departmento de Ciências Veterinárias, Centro de Ciências Agrárias, Universidade Federal da Paraíba; Departmento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina
Departmento de Ciências Veterinárias, Centro de Ciências Agrárias, Universidade Federal da Paraíba; Departmento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina
How to Cite
Vieira, R., Vieira, T., Nascimento, D. do, Martins, T., Krawczak, F., Labruna, M., Chandrashekar, R., Marcondes, M., Biondo, A., & Vidotto, O. (1). SEROLOGICAL SURVEY OF Ehrlichia SPECIES IN DOGS, HORSES AND HUMANS: ZOONOTIC SCENERY IN A RURAL SETTLEMENT FROM SOUTHERN BRAZIL. Revista Do Instituto De Medicina Tropical De São Paulo, 55(5), 335-340. Retrieved from http://www.revistas.usp.br/rimtsp/article/view/78883
Section
Microbiology