Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a

  • Flávia Mandolesi Pereira de Melo Embrapa Meio Ambiente; Lab. de Microbiologia Ambiental
  • Marli Fátima Fiore USP; CENA; Lab. de Biologia Celular e Molecular
  • Luiz Alberto Beraldo de Moraes USP; FFCLRP; Depto. de Química
  • Maria Estela Silva-Stenico USP; CENA; Lab. de Biologia Celular e Molecular
  • Shirlei Scramin Embrapa Meio Ambiente; Lab. de Microbiologia Ambiental
  • Manoel de Araújo Teixeira UNIVÁS; Lab. de Microbiologia
  • Itamar Soares de Melo Embrapa Meio Ambiente; Lab. de Microbiologia Ambiental
Keywords: ESI-MS/MS, pumilacidin, endophytic bacteria, bioautography

Abstract

In the search for new organisms and new secondary metabolites, a study was conducted to evaluate the diversity of endophytic bacteria from ethnovarieties of cassava cultivated by Brazilian Amazon Indian tribes and also to study the secondary metabolites produced by a Bacillus pumilus strain. Sixty seven cassava endophytic bacteria were subjected to 16S rRNA sequencing and FAME analysis. The bacterial profile revealed that 25% of all endophytic isolates belonged to the genus Bacillus. The isolate B. pumilus MAIIIM4a showed a strong inhibitory activity against the fungi Rhizoctonia solani, Pythium aphanidermatum and Sclerotium rolfsii. Secondary metabolites of this strain were extracted using hexane, dichloromethane and ethyl acetate. Extracts were subjected to bioautography and LC/MS analysis, which allowed the identification of pumilacidin, an antifungal compound produced by B. pumilus MAIIIM4a. The bacterial endophytic localization was confirmed by cassava cell tissue examination using scanning electron microscopy.

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Published
2009-10-01
How to Cite
Melo, F., Fiore, M., Moraes, L., Silva-Stenico, M., Scramin, S., Teixeira, M., & Melo, I. (2009). Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a . Scientia Agricola, 66(5), 583-592. https://doi.org/10.1590/S0103-90162009000500002
Section
Agricultural Microbiology