Stir bar-sorptive extraction, solid phase extraction and liquid-liquid extraction for levetiracetam determination in human plasma: comparing recovery rates

Authors

  • Priscila Freitas-Lima Universidade de São Paulo; School of Medicine of Ribeirão Preto
  • Flavia Isaura Santi Ferreira Universidade de São Paulo; Faculty of Pharmaceutical Sciences of Ribeirão Preto
  • Carlo Bertucci Università di Bologna; Department of Pharmacy and Biotechnology
  • Veriano Alexandre Júnior Universidade de São Paulo; School of Medicine of Ribeirão Preto
  • Sônia Aparecida Carvalho Dreossi Universidade de São Paulo; Faculty of Pharmaceutical Sciences of Ribeirão Preto
  • Leonardo Regis Leira Pereira Universidade de São Paulo; Faculty of Pharmaceutical Sciences of Ribeirão Preto
  • Américo Ceiki Sakamoto Universidade de São Paulo; School of Medicine of Ribeirão Preto
  • Regina Helena Costa Queiroz Universidade de São Paulo; Faculty of Pharmaceutical Sciences of Ribeirão Preto

DOI:

https://doi.org/10.1590/S1984-82502015000200017

Abstract

Levetiracetam (LEV), an antiepileptic drug (AED) with favorable pharmacokinetic profile, is increasingly being used in clinical practice, although information on its metabolism and disposition are still being generated. Therefore a simple, robust and fast liquid-liquid extraction (LLE) followed by high-performance liquid chromatography method is described that could be used for both pharmacokinetic and therapeutic drug monitoring (TDM) purposes. Moreover, recovery rates of LEV in plasma were compared among LLE, stir bar-sorptive extraction (SBSE), and solid-phase extraction (SPE). Solvent extraction with dichloromethane yielded a plasma residue free from usual interferences such as commonly co-prescribed AEDs, and recoveries around 90% (LLE), 60% (SPE) and 10% (SBSE). Separation was obtained using reverse phase Select B column with ultraviolet detection (235 nm). Mobile phase consisted of methanol:sodium acetate buffer 0.125 M pH 4.4 (20:80, v/v). The method was linear over a range of 2.8-220.0 µg mL;-1;. The intra- and inter-assay precision and accuracy were studied at three concentrations; relative standard deviation was less than 10%. The limit of quantification was 2.8 µg mL;-1;. This robust method was successfully applied to analyze plasma samples from patients with epilepsy and therefore might be used for pharmacokinetic and TDM purposes.

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Published

2015-06-01

How to Cite

Freitas-Lima, P., Ferreira, F. I. S., Bertucci, C., Alexandre Júnior, V., Dreossi, S. A. C., Pereira, L. R. L., Sakamoto, A. C., & Queiroz, R. H. C. (2015). Stir bar-sorptive extraction, solid phase extraction and liquid-liquid extraction for levetiracetam determination in human plasma: comparing recovery rates. Brazilian Journal of Pharmaceutical Sciences, 51(2), 393-401. https://doi.org/10.1590/S1984-82502015000200017

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Articles