Statistical optimization of dithranol-loaded solid lipid nanoparticles using factorial design
DOI:
https://doi.org/10.1590/S1984-82502011000300008Keywords:
Solid lipid nanoparticles, Homogenization, Ultrasonication, 3² factorial design, DithranolAbstract
This study describes a 3² full factorial experimental design to optimize the formulation of dithranol (DTH) loaded solid lipid nanoparticles (SLN) by the pre-emulsion ultrasonication method. The variables drug: lipid ratio and sonication time were studied at three levels and arranged in a 3² factorial design to study the influence on the response variables particle size and % entrapment efficiency (%EE). From the statistical analysis of data polynomial equations were generated. The particle size and %EE for the 9 batches (R1 to R9) showed a wide variation of 219-348 nm and 51.33- 71.80 %, respectively. The physical characteristics of DTH-loaded SLN were evaluated using a particle size analyzer, differential scanning calorimetry and X-ray diffraction. The results of the optimized formulation showed an average particle size of 219 nm and entrapment efficiency of 69.88 %. Ex-vivo drug penetration using rat skin showed about a 2-fold increase in localization of DTH in skin as compared to the marketed preparation of DTH.Downloads
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Published
2011-09-01
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Statistical optimization of dithranol-loaded solid lipid nanoparticles using factorial design . (2011). Brazilian Journal of Pharmaceutical Sciences, 47(3), 503-511. https://doi.org/10.1590/S1984-82502011000300008