Effect of solvent polarity on extraction yield and antioxidant properties of phytochemicals from bean (Phaseolus vulgaris) seeds

Authors

  • Haq Nawaz Bahauddin Zakariya University, Department of Biochemistry
  • Muhammad Aslam Shad Bahauddin Zakariya University, Department of Biochemistry
  • Najiha Rehman Bahauddin Zakariya University, Institute of Chemical Sciences
  • Hina Andaleeb Bahauddin Zakariya University, Department of Biochemistry
  • Najeeb Ullah Bahauddin Zakariya University, Department of Biochemistry

DOI:

https://doi.org/10.1590/s2175-97902019000417129

Keywords:

Beans seeds/antioxidant properties, Phaseolus vulgaris/phytochemical composition, Regression analysis, Solvent polarity

Abstract

The effect of solvent polarity on extraction yield and antioxidant properties of phytochemical compounds in bean seeds was studied. Seed flour of three varieties of bean was extracted in a series of organic solvents with increasing polarity (n-hexane, petroleum ether, chloroform, ethyl acetate, ethanol, acetone and water). Preliminary screening of phytochemicals showed the presence of tannins, flavonoids, cardiac glycosides, anthocyanins, terpenoids, carotenoids, ascorbic acid and reducing compounds in all extracts. One way analysis of variance (ANOVA) of results showed that extraction yield, phytochemical content and antioxidant properties were significantly influenced (p<0.05) by the polarity of extracting solvents. The regression analysis of data showed polarity-dependent second order polynomial variations in the extraction yield, phytochemical contents, antioxidant activity, reducing properties and free radical scavenging activity of each variety. Extraction in highly polar solvents resulted in high extract yield but low phenolic and flavonoid content as compared to non-polar ones. The polarity-dependent increase in total antioxidant activity and reducing properties indicates the extraction of strong antioxidant compounds in polar solvents. The study suggests the use of a combination of polar and nonpolar solvents to increase the extraction efficiency of phytochemicals with good antioxidant quality from the bean and other legume seeds.

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Published

2020-12-09

How to Cite

Nawaz, H. ., Shad, M. A. ., Rehman, N. ., Andaleeb, H. ., & Ullah, N. . (2020). Effect of solvent polarity on extraction yield and antioxidant properties of phytochemicals from bean (Phaseolus vulgaris) seeds. Brazilian Journal of Pharmaceutical Sciences, 56, e17129. https://doi.org/10.1590/s2175-97902019000417129

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