Oleanolic acid from black raisins, Vitis vinifera with antioxidant and antiproliferative potentials on HCT 116 colon cancer cell line

Authors

  • Kandasamy Sasikumar VIT University, School of Bio Sciences and Technology, Department of Integrative Biology
  • Vinay Dubey VIT University, School of Bio Sciences and Technology, Department of Integrative Biology
  • Asit Ranjan Ghosh VIT University, School of Bio Sciences and Technology, Department of Integrative Biology https://orcid.org/0000-0002-7922-3308

DOI:

https://doi.org/10.1590/s2175-97902019000417158

Keywords:

Vitis vinifera, Oleanolic acid, Anti-oxidant, Anti-proliferative

Abstract

Vitis vinifera (black raisin) is commonly used in traditional medicine for the treatment of various ailments. In the present study, anti-oxidative and anti-cancer efficacy of oleanolic acid from ethyl acetate fraction of black raisins was evaluated and oleanolic acid was isolated without using of any chromatographic techniques and subjected to spectral assessment using UV-Vis spectrophotometer, 1 H NMR, 13C NMR, MS and FT-IR for structural confirmation. Antiproliferative efficacy of oleanolic acid against human colon adenocarcinoma HCT-116 cells was assessed using cell viability assay. The minimum inhibitory concentration (IC50) was determined and found to be 40 µg/mL at 48h incubation. Furthermore, antioxidant property of oleanolic acid was analyzed using DPPH method (IC50 is 61.5µg/mL) by compared to standard antioxidants ascorbic acid, gallic acid, pyrogallol and butylated hydroxytoluene. Hence, the present study aims to establish the use of oleanolic acid as a potential therapeutic agent against human colon cancer.

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Published

2020-12-09

How to Cite

Sasikumar, K. ., Dubey, V. ., & Ghosh, A. R. . (2020). Oleanolic acid from black raisins, Vitis vinifera with antioxidant and antiproliferative potentials on HCT 116 colon cancer cell line. Brazilian Journal of Pharmaceutical Sciences, 56, e17158. https://doi.org/10.1590/s2175-97902019000417158

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