Quantification of chlorogenic acid, rosmarinic acid, and caffeic acid contents in selected Thai medicinal plants using RP-HPLC-DAD

Authors

  • Chayanon Chaowuttikul Chulalongkorn University, College of Public Health Sciences
  • Chanida Palanuvej Chulalongkorn University, College of Public Health Sciences https://orcid.org/0000-0003-2688-8784
  • Nijsiri Ruangrungsi Chulalongkorn University, College of Public Health Sciences

DOI:

https://doi.org/10.1590/s2175-97902019000317547

Keywords:

Chlorogenic acid, Rosmarinic acid, Caffeic acid, RP-HPLC-DAD, Medicinal plant

Abstract

The chlorogenic acid, rosmarinic acid, and caffeic acid contents in 100 selected plants were determined using reversed phase high performance liquid chromatography equipped with diode array detector. The optimum condition was 0.2% phosphoric acid in water (solvent A) and methanol (solvent B) as the mobile phase, which was set at 45% B for 20 minutes at a flow rate of 1.2 mL/min. The column temperature was maintained at 30 °C and the detection wavelength was 325 nm. Among 100 selected plants, 39.64% contained all 3 compounds, 40.54% contained 2 compounds, 14.41% contained only 1 compound, and 5.41% could not detect any of the 3 compounds. The highest contents of chlorogenic acid, rosmarinic acid, and caffeic acid were found in Lonicera japonica flowering buds, Melissa officinalis leaves, and Coffea canephora seeds at the concentration of 9.900 ± 0.004, 19.908 ± 0.171, and 1.233 ± 0.003 g/100 g of dried plant, respectively.

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Published

2020-12-09

How to Cite

Chaowuttikul, C. ., Palanuvej, C. ., & Ruangrungsi, N. . (2020). Quantification of chlorogenic acid, rosmarinic acid, and caffeic acid contents in selected Thai medicinal plants using RP-HPLC-DAD. Brazilian Journal of Pharmaceutical Sciences, 56, e17547. https://doi.org/10.1590/s2175-97902019000317547

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