Chiral discrimination of amlodipine from pharmaceutical products using capillary electrophoresis

Authors

  • Melania Cârcu-Dobrin University of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Chemistry
  • Alexandra Georgiana Sabău University of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Chemistry
  • Gabriel Hancu University of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Chemistry https://orcid.org/0000-0003-2564-9271
  • Gyéresi Árpád University of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Chemistry
  • Aura Rusu University of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Chemistry https://orcid.org/0000-0003-4979-6903
  • Hajnal Kelemen University of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Chemistry https://orcid.org/0000-0001-5043-3937
  • Lajos Attila Papp University of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Chemistry
  • Anca Cârje University of Medicine and Pharmacy, Faculty of Pharmacy, Department of Analytical Chemistry and Drug Analysis

DOI:

https://doi.org/10.1590/s2175-97902019000318259

Keywords:

Amlodipine, Capillary electrophoresis, Chiral separation, Cyclodextrine, Experimental design

Abstract

The present work describes the development of a capillary electrophoresis (CE) method for the chiral discrimination of amlodipine (AML) enantiomers using cyclodextrine (CD) derivatives as chiral selectors. A large number of native and derivatized, neutral and ionized CD derivatives were screened to find the optimal chiral selector; and carboximethyl-β-CD (CM-β-CD) was selected for the enantiomeric discrimination. A factorial analysis study was performed by orthogonal experimental design in which several factors were varied at the same time to optimize the separation method. The optimized method (25 mM phosphate buffer, pH = 9.0, 15 mM CM-β-CD, 15 °C, + 25 kV, 30 mbar/1 second, detection wavelength 230 nm) was successfully applied for the baseline separation of AML enantiomers within 5 minutes. Successful validation and application of the proposed CE method suggest its routine use in enantioselective control of AML in pharmaceutical preparations.

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Published

2020-12-09

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Article

How to Cite

Chiral discrimination of amlodipine from pharmaceutical products using capillary electrophoresis. (2020). Brazilian Journal of Pharmaceutical Sciences, 56, e18259. https://doi.org/10.1590/s2175-97902019000318259