Determination of phenolic and triterpenic compounds in Jatropha gossypiifolia L by Ultra-high performance liquid chromatography-tandem mass spectrometric (UHPLC-MS/MS)

Authors

  • Rudinei da Silva Silveira Federal University of Santa Maria, Pharmaceutical Sciences Graduate Program
  • Gabriela Camera Leal Federal University of Santa Maria, Pharmaceutical Sciences Graduate Program
  • Thais Ramos Dal Molin Federal University of Santa Maria, Pharmaceutical Sciences Graduate Program
  • Henrique Faccin Federal University of Santa Maria, Department of Chemistry
  • Luciana Assis Gobo Federal University of Santa Maria, Department of Chemistry
  • Géssica Domingos da Silveira Federal University of Santa Maria, Department of Chemistry
  • Mariana Tatsch da Silva Souza Federal University of Santa Maria, Center of Health Sciences, Pharmacy Faculty
  • Osmar Alves Lameira EMBRAPA Amazônia Oriental, Laboratory of Agrobiotechnology
  • Leandro Machado de Carvalho Federal University of Santa Maria, Pharmaceutical Sciences Graduate Program
  • Carine Viana Federal University of Santa Maria, Pharmaceutical Sciences Graduate Program https://orcid.org/0000-0001-9069-1076

DOI:

https://doi.org/10.1590/s2175-97902019000417262

Keywords:

Jatropha gossypiifolia L, UHPLC-MS/MS, Phenolic compounds, Triterpenic compounds

Abstract

This work describes the chemical characterization of extracts of Jatropha gossypiifolia (from Amazonia region) concerning the presence of phenolic and triterpenic compounds using Ultra High Perfomance Liquid chromatography coupled to Mass Spectrometry in Tandem (UHPLC-MS/MS) methods. The studied compounds belong to the most frequently found classes in medicinal plants (triterpenes, flavonoids, flavones, hydroxycinnamic acids, coumarins, catechins and stilbenes), which have been analyzed as chemical and bioactive markers in hydroethanolic and aqueous extracts. Several polyphenolic compounds identified herein are unprecedented in the scientific literature for this species. The chemical markers identified and quantified in the studied extracts of J. gossypiifolia were gallic acid, chlorogenic acid, catechin, caffeic acid, vanillic acid, p-coumaric acid, ferullic acid, rutin, quercitrin, 3-acetylcoumarin, trans-cinnamic acid, quercetin, luteolin, apigenin, kaempferol, chrysin. α-amyrin, β-amyrin and lupeol. As the majority compounds, (+)-catechin, p-coumaric acid, ferulic acid, luteolin, α-amyrin and β-amyrin were found to be present at mg kg−1 levels. J. gossypiifolia extracts presented a high in vitro activity against different reactive oxygen species (hydroxyl, peroxyl, and superoxide anion radicals). Several polyphenolic compound data presented herein are unprecedented in the scientific literature for this plant species. As a result, this plant can be a new source of bioactive molecules for therapeutic purposes.

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Published

2020-12-09

How to Cite

Silveira, R. da S. ., Leal, G. C. ., Molin, T. R. D. ., Faccin, H. ., Gobo, L. A. ., Silveira, G. D. da ., Souza, M. T. da S. ., Lameira, O. A. ., Carvalho, L. M. de ., & Viana, C. . (2020). Determination of phenolic and triterpenic compounds in Jatropha gossypiifolia L by Ultra-high performance liquid chromatography-tandem mass spectrometric (UHPLC-MS/MS) . Brazilian Journal of Pharmaceutical Sciences, 56, e17262. https://doi.org/10.1590/s2175-97902019000417262

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