Anti-inflammatory activity and identification of the Verbena litoralis Kunth crude extract constituents

Authors

  • Rachel de Lima Federal University of Santa Maria, Post-Graduate Program in Pharmaceutical Sciences
  • Juliana Calil Brondani Federal University of Santa Maria, Post-Graduate Program in Pharmaceutical Sciences https://orcid.org/0000-0003-3349-3449
  • Rafaela Castro Dornelles Federal University of Santa Maria, PostGraduate Program in Pharmacology
  • Cibele Lima Lhamas Federal University of Santa Maria, Veterinary Hospital
  • Henrique Faccin Federal University of Santa Maria, Chemistry Department
  • Carine Viana Silva Federal University of Santa Maria, Post-Graduate Program in Pharmaceutical Sciences
  • Sérgio Luiz Dalmora Federal University of Santa Maria, Post-Graduate Program in Pharmaceutical Sciences
  • Melânia Palermo Manfron Federal University of Santa Maria, Post-Graduate Program in Pharmaceutical Sciences

DOI:

https://doi.org/10.1590/s2175-97902019000417419

Keywords:

Verbena litoralis, UHPLC/MS, Flavonoids, Anti-inflammatory

Abstract

Verbena litoralis is a plant popularly known as “gervãozinho-do-campo” in Portuguese. It is traditionally used for stomach, liver and gallbladder problems, and as an anti-inflammatory and anthelmintic. The goal of this study was to determine the chemical composition of the crude extract obtained from the aerial parts of V. litoralis by Ultra High Performance Liquid chromatography coupled with Mass Spectrometry in Tandem (UHPLC-MS/MS); assess the anti-inflammatory activity of ethyl acetate fraction and of crude extract; and verify liver, kidney and pancreas damage. In this study, the chemical composition of the extract was identified via UHPLC/MS/MS, assessing the anti-inflammatory activity of the crude extract and the acetate fraction in an induction model of the granulomatous tissue, as well as given liver, kidney and pancreas damage markers. Chlorogenic acid, luteolin, caffeic acid, apigenin, p-coumaric acid, vanillic acid, ferulic acid and quercetin were quantified in the extract. After the seven-day treatment, the granuloma of the animals treated with the plant extract and fraction presented values very close to the positive control (nimesulide). The V. litoralis crude extract and ethyl acetate fraction show antiinflammatory activity similar to the nimesulide without evidence of liver, kidney and pancreas damage, which attributes the plant’s pharmacological action to the flavonoids found.

Downloads

Download data is not yet available.

Downloads

Published

2020-12-09

How to Cite

Lima, R. de ., Brondani, J. C. ., Dornelles, R. C. ., Lhamas, C. L. ., Faccin, H. ., Silva, C. V. ., Dalmora, S. L. ., & Manfron, M. P. . (2020). Anti-inflammatory activity and identification of the Verbena litoralis Kunth crude extract constituents. Brazilian Journal of Pharmaceutical Sciences, 56, e17419. https://doi.org/10.1590/s2175-97902019000417419

Issue

Section

Article