Utilization of [Bmim]BF4-MAE on enhancement of γ-oryzanol extraction from rice bran and its tyrosinase inhibitory activity

Authors

  • Arif Arrahman Universitas Indonesia, Faculty of Pharmacy, Department of Pharmaceutical-Medicinal Chemistry and Bioanalysis https://orcid.org/0000-0002-6596-2847
  • Efendy Sigalingging Universitas Indonesia, Faculty of Pharmacy, Department of Pharmacognosy and Phytochemistry
  • Elsa Trinovita Universitas Indonesia, Faculty of Pharmacy, Graduate Program of Herbal Medicine
  • Fadlina Chany Saputri Universitas Indonesia, Faculty of Pharmacy, Department of Pharmacology and Toxycology
  • Abdul Mun’im

DOI:

https://doi.org/10.1590/s2175-97902019000418115

Keywords:

[Bmim]BF4. Ionic liquid, IL-MAE, γ-oryzanol, Tyrosinase

Abstract

This study investigated the effect of ionic liquid (IL)-microwave assisted extraction (MAE) on γ-oryzanol extraction from rice bran (Oryza sativa L.). The conditions included the concentration of IL, liquid (IL)/ solid (sample) ratio, extraction time, and microwave power. The use of 1-butyl-3-methylimidazolium ([Bmim]BF4) as a selected solvent in IL-MAE method was developed for extraction of γ-oryzanol from rice bran. The Box-Behnken design on four factors with response surface methodology (RSM) was used to optimize the experimental conditions. The optimum conditions for the MAE were a [Bmim]BF4 concentration of 0.7 M, liquid/solid ratio of 15 mL/g, extraction time of 10 minutes, and 30% microwave power with a γ-oryzanol value of 0.41 mg/g. The experimental values agreed with those predicted by the RSM models. A microplate reader method was used to determine the IC50 value in tyrosinase inhibitory activity of the extract. An IC50 value of the extract obtained from IL-MAE was 1240 µg/mL. This study suggests that IL-MAE is suitable for the extraction of γ-oryzanol from rice bran and that under the optimum conditions the extract has low activity as a tyrosinase inhibitor.

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Published

2020-12-09

How to Cite

Arrahman, A. ., Sigalingging, E. ., Trinovita, E. ., Saputri, F. C. ., & Mun’im, A. . (2020). Utilization of [Bmim]BF4-MAE on enhancement of γ-oryzanol extraction from rice bran and its tyrosinase inhibitory activity. Brazilian Journal of Pharmaceutical Sciences, 56, e18115. https://doi.org/10.1590/s2175-97902019000418115

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