The in vitro and in vivo investigation of biological activities and phenolic analysis of Helichrysum plicatum subsp. plicatum

Authors

  • Turgut Taşkın Marmara University, Faculty of Pharmacy, Department of Pharmacognosy https://orcid.org/0000-0001-8475-6478
  • Tuğba Gezmiş Aysu Pharmacy
  • Muhammet Emin Çam Marmara University, Faculty of Pharmacy, Department of Pharmacology
  • Duygu Taşkın University of Health Sciences, Faculty of Pharmacy, Department of Analytical Chemistry
  • Berna Özbek Çelik Istanbul University, Faculty of Pharmacy, Department of Pharmaceutical Microbiology
  • İsmail Şenkardeş Marmara University, Faculty of Pharmacy, Department of Pharmaceutical Botany
  • Sevda Süzgeç-Selçuk Istanbul University, Faculty of Pharmacy, Department of Pharmacognosy

DOI:

https://doi.org/10.1590/s2175-97902019000418345

Keywords:

Helichrysum plicatum subsp, plicatum, Anti-inflammatory, Anti-urease, Anticholinesterase, HPLC-DAD-MS

Abstract

In Turkey, Helichrysum genus is represented by 26 taxa belonging to 20 species in Turkish flora of which 14 ones are endemic to Turkey. The aerial parts of Helichrysum plicatum subsp. plicatum are used kidney stones, kidney and stomach ailments. The extraction procedures and solvents are important step in processing of bioactive constituents from the plant materials. Therefore, the aim of this study was to evaluate in vitro antioxidant, antimicrobial, anti-urease, anticholinesterase and in vivo antiinflammatory activities of Helichrysum plicatum subsp. plicatum different extracts. In addition, the phenolic characterization of the Soxhlet and maceration methanol extracts which showed significant antioxidant, anti-urease, antimicrobial, anti-inflammatory and anticholinesterase activities were performed by HPLC-DAD and LC-MS/MS. In the present study, the Soxhlet methanol extract exhibited strong antioxidant, antimicrobial and anticholinesterase activities than other extracts. The maceration methanol extract showed the strongest anti-urease activity. The Soxhlet methanol and maceration methanol extracts showed in vivo anti-inflammatory activities very close to each other. As a result of this study, chlorogenic acid, dicaffeoylquinic acid, luteolin, luteolin-7-O-glucoside, naringenin-O-hexoside and isoquercitrin compounds were analysed in plant. Therefore, it is thought that methanol extracts can be used as a natural source in the future for food and pharmaceutical industries.

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Published

2020-12-09

How to Cite

Taşkın, T. ., Gezmiş, T. ., Çam, M. E. ., Taşkın, D. ., Çelik, B. Özbek ., Şenkardeş, İsmail ., & Süzgeç-Selçuk, S. . (2020). The in vitro and in vivo investigation of biological activities and phenolic analysis of Helichrysum plicatum subsp. plicatum. Brazilian Journal of Pharmaceutical Sciences, 56, e18345. https://doi.org/10.1590/s2175-97902019000418345

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