Polymeric micelles containing resveratrol: development, characterization, cytotoxicity on tumor cells and antimicrobial activity

Authors

  • Tamires Cunha Almeida Universidade Federal de Ouro Preto, Departamento de Análises Clínicas, Laboratório de Pesquisas Clínicas https://orcid.org/0000-0002-5584-3609
  • Janaina Brandão Seibert Universidade Federal de Ouro Preto, Departamento de Farmácia, Laboratório de Fitotecnologia
  • Sávio Henrique de Souza Almeida Universidade Federal de Ouro Preto, Departamento de Análises Clínicas, Laboratório de Pesquisas Clínicas
  • Tatiane Roquete Amparo Universidade Federal de Ouro Preto, Departamento de Farmácia, Laboratório de Fitotecnologia
  • Luiz Fernando de Medeiros Teixeira Universidade Federal de Ouro Preto, Departamento de Análises Clínicas, Laboratório de Microbiologia
  • Jose Mario Barichello Universidade Federal de Pelotas, Farmacêuticas e de Alimentos, Centro de Ciências Químicas, Laboratório de Tecnologia Farmacêutica
  • Bruna Bueno Postacchini Universidade Federal de Ouro Preto, Departamento de Física, Laboratório de Fotofísica Molecular
  • Orlando David Henrique dos Santos Universidade Federal de Ouro Preto, Departamento de Farmácia, Laboratório de Fitotecnologia
  • Glenda Nicioli da Silva Universidade Federal de Ouro Preto, Departamento de Análises Clínicas, Laboratório de Pesquisas Clínicas

DOI:

https://doi.org/10.1590/s2175-97902019000418401

Keywords:

Antimicrobial activity, Cytotoxic activity, F127®, Polymeric micelles, Resveratrol

Abstract

Antimicrobial and antitumor activities of resveratrol, a compound found mainly in grapes, have already been demonstrated. However, its low bioavailability is a limiting factor for therapeutic application. Polymeric micelles can be an approach to solve this problem since they can encapsulate hydrophobic substances. We developed and characterized micellar formulations containing resveratrol and evaluated their cytotoxic and antimicrobial effects. The formulations were prepared by the cold dispersion method with different concentrations of F127 (5 or 10% w/w) and resveratrol (500 or 5000 µM). The formulations were characterized according to size, polydispersity index, pH, encapsulation rate and in vitro release. Cytotoxic effect was evaluated on a bladder cancer cell line and antimicrobial effect was evaluated on E. coli, S. aureus and C. albicans. One of the formulations (10% w/w of F127 and 5000 µM of resveratrol) was a monodispersed solution with high encapsulation rate, thus it was chosen for the cytotoxicity and antimicrobial assays. MS10+RES-3 was able to preserve the antimicrobial and cytotoxic activity of resveratrol. This is the first study that evaluated antimicrobial potential and cytotoxicity of micelles containing resveratrol on bladder cancer cells and the results showed that micellar nanostructures could ensure the maintenance of the biological activity of resveratrol.

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Published

2020-12-09

How to Cite

Almeida, T. C. ., Seibert, J. B. ., Almeida, S. H. de S. ., Amparo, T. R. ., Teixeira, L. F. de M. ., Barichello, J. M. ., Postacchini, B. B. ., Santos, . O. D. H. dos ., & Silva, G. N. da . (2020). Polymeric micelles containing resveratrol: development, characterization, cytotoxicity on tumor cells and antimicrobial activity. Brazilian Journal of Pharmaceutical Sciences, 56, e18411. https://doi.org/10.1590/s2175-97902019000418401

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