Development and validation of highly selective method for the determination of imatinib mesylate and dexketoprofen trometamol combination in three different media

Authors

  • Ozlem Coban Karadeniz Technical University Faculty of Pharmacy, Department of Pharmaceutical Technology
  • Zelihagul Degim Biruni University Faculty of Pharmacy, Department of Pharmaceutical Technology https://orcid.org/0000-0003-2596-3615

DOI:

https://doi.org/10.1590/s2175-97902019000418583

Keywords:

Imatinib mesylate, Dexketoprofen trometamol, Ultra Performance Liquid Chromatography, Dulbecco’s Modified Eagle Medium

Abstract

Imatinib mesylate is a small molecule used in cancer therapy as a thyrosine kinase inhibitor. Dexketoprofen trometamol is a non-steroidal anti-inflammatory drug that has seen use in cancer therapy in combination with an anticancer drug to minimize tumor size and to reduce pain in patients. In the present study, imatinib mesylate and dexketoprofen trometamol were selected as potential model drugs to be used in combination. A new, simple and selective Ultra Performance Liquid Chromatography method was developed and validated to determine the drug substances in distilled water, in a pH 7.4 phosphate buffer and in Dulbecco’s Modified Eagle Medium. The proposed method was developed using a BEH C-18 column with isocratic elution. A mixture of methanol:acetonitrile (80:20, v/v) and pH 9.5, 0.05 M ammonium acetate were (70:30, v/v) used as a mobile phase. Detection was carried out with a flow rate of 0.3 mL/min, a column temperature of 30°C and an injection volume of 20 µL. The method was validated considering linearity, accuracy, precision, specificity, robustness, detection limit and quantitation limit values, and was found to be linear in a range from 0.05 to 20.0 µg/mL for the three different media.

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Published

2020-12-09

How to Cite

Coban, O. ., & Degim, Z. . (2020). Development and validation of highly selective method for the determination of imatinib mesylate and dexketoprofen trometamol combination in three different media. Brazilian Journal of Pharmaceutical Sciences, 56, e18583. https://doi.org/10.1590/s2175-97902019000418583

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