Preparation and in vitro characterization of monoclonal antibody ranibizumab conjugated magnetic nanoparticles for ocular drug delivery

Authors

  • Neşe Ayata Marmara University, Faculty of Pharmacy, Department of Pharmaceutical Biotechnology
  • Ali Demir Sezer Marmara University, Faculty of Pharmacy, Department of Pharmaceutical Biotechnology https://orcid.org/0000-0002-2678-8903
  • Seyda Bucak Yeditepe University, Faculty of Engineering, Department of Chemical Engineering
  • Eda Tahir Turanlı İstanbul Technical University, Faculty of Science and Letters, Department of Molecular Biology and Genetics

DOI:

https://doi.org/10.1590/s2175-97902020000118171

Keywords:

Ranibizumab, Monoclonal antibody, Drug delivery, Magnetic nanoparticles, Cell culture

Abstract

Gold coated magnetite nanoparticles were prepared and coated with ranibizumab as an ocular drug delivery system. The surface morphologies of the nanoparticles were determined by Scanning Electron Microscopy (SEM). The size and surface charge were determined by using the dynamic light scattering (DLS) technique. Crystallographic properties of the gold coated Fe3 O4 nanoparticles were recorded on X-ray diffractometer (XRD) the XRD pattern of nanoparticlees were shown to have uniqe Fe3 O4 and gold peaks.Conjugation of ranibizumab onto nanoparticles was achieved using the physical adsorption method. The amount of ranibizumab on the surface of the nanoparticles was determined by thermogravimetric analysis (TGA). In the in vitro release studies performed using UV spectroscopy; it was found that almost 60% of antibodies were released within the first 30 minutes. Antibody activity after release studies was also proved with ELISA. Non-toxicity of gold coated Fe3 O4 particles were proved with MTT. Results of the studies, showed that the antibody conjugated magnetic nanoparticle system could be a potential treatment system for ocular diseases.

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Published

2020-12-09

How to Cite

Ayata, N. ., Sezer, A. D. ., Bucak, S. ., & Turanlı, E. T. . (2020). Preparation and in vitro characterization of monoclonal antibody ranibizumab conjugated magnetic nanoparticles for ocular drug delivery. Brazilian Journal of Pharmaceutical Sciences, 56, e18171. https://doi.org/10.1590/s2175-97902020000118171

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