Development, in vitro biocompatibility, and antitumor efficacy of acetic acid-modified Cordyceps sinensis polysaccharide nanoparticle drug delivery system

Authors

  • Jiao Guan Jilin Medical University, School of Pharmacy
  • Liqin Han Jilin Medical University, School of Pharmacy
  • Nianqiu Shi Jilin Medical University, School of Pharmacy
  • Heyun Zhu Jilin Medical University, School of Pharmacy https://orcid.org/0000-0003-0212-0121
  • Junmin Wang Jinlin Medical University, Affiliated Hospital

DOI:

https://doi.org/10.1590/s2175-97902019000418470

Keywords:

Docetaxel, Cordyceps sinensis polysaccharide, Nanoparticles, Antitumor efficacy, Biocompatibility

Abstract

Docetaxel-loaded acetic acid conjugated Cordyceps sinensis polysaccharide (DTX-AA-CSP) nanoparticles were prepared through dialysis and their release rates in vitro, particle sizes, zeta potentials, drug loading capacities, and encapsulation efficiencies were characterized for the synthesis of AA-modified CSPs from traditional Chinese medicine Cordyceps sinensis (Berk.) Sacc. Then, the AA-modified CSPs were characterized by 1 H-NMR and FT-IR. Furthermore, the biocompatibility of the delivery carrier (AA-CSP nanoparticles) was assessed on human umbilical vein endothelial cells. In vitro antitumor activity studies on DTX-AA-CSP nanoparticles were conducted on the human liver (HepG2) and colon cancer cells (SW480). The DTX-AA-CSP nanoparticles were spherical and had an average size of 98.91±0.29 nm and zeta potential within the −19.75±1.13 mV. The encapsulation efficiency and loading capacity were 80.95%±0.43% and 8.09%±0.04%, respectively. In vitro, DTX from the DTX-AA-CSP nanoparticles exhibited a sustained release, and the anticancer activities of DTX-AA-CSP nanoparticles against SW480 and HepG2 were significantly higher than those of marketed docetaxel injection (Taxotere®) in nearly all the tested concentrations. The AA-CSP nanoparticles showed good biocompatibility. This study provided a promising biocompatible delivery system for carrying antitumor drugs for cancer therapy.

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Published

2020-12-09

How to Cite

Guan, J. ., Han, L., Shi, N. ., Zhu, H. ., & Wang, J. . (2020). Development, in vitro biocompatibility, and antitumor efficacy of acetic acid-modified Cordyceps sinensis polysaccharide nanoparticle drug delivery system. Brazilian Journal of Pharmaceutical Sciences, 56, e18470 . https://doi.org/10.1590/s2175-97902019000418470

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