Potentially pathogenic bacteria isolated from neglected air and surfaces in hospitals

Mayk Teles de Oliveira; Lorranny Mayara  Silva Cunha; Fernanda Cardoso Cruz; Nathany Kelly Ribeiro Batista; Eric de Souza Gi; Virgínia Farias Alves; Maria Teresa Freitas Bara; Ieda Maria Sapateiro Torres

doi:10.1590/s2175-97902020000418989

Authors

Mayk Teles de Oliveira Universidade Federal de Goias Ringgold standard institution - Faculty of Pharmacy Goiania, GO Brazi
Lorranny Mayara Silva Cunha Research Laboratory of Medicines and Food Microbiology, Faculty of Pharmacy, Federal University of Goiás, Goiânia, Goiás, Brazil
Fernanda Cardoso Cruz Research Laboratory of Medicines and Food Microbiology, Faculty of Pharmacy, Federal University of Goiás, Goiânia, Goiás, Brazil
Nathany Kelly Ribeiro Batista Research Laboratory of Medicines and Food Microbiology, Faculty of Pharmacy, Federal University of Goiás, Goiânia, Goiás, Brazil
Eric de Souza Gi Research Laboratory of Pharmaceutical and Environmental Analysis, Faculty of Pharmacy, Federal University of Goiás, Goiânia, Goiás, Brazil
Virgínia Farias Alves Research Laboratory of Medicines and Food Microbiology, Faculty of Pharmacy, Federal University of Goiás, Goiânia, Goiás, Brazil
Maria Teresa Freitas Bara Research Laboratory of Natural Products, Faculty of Pharmacy, Federal University of Goiás, Goiânia, Goiás, Brazil
Ieda Maria Sapateiro Torres Universidade Federal de Goias Ringgold standard institution Rua Samuel Morse, 21 Apto 1304, Ed. Sky Life, Goiania 74835080 Brazil https://orcid.org/0000-0001-8407-627X

DOI:

https://doi.org/10.1590/s2175-97902020000418989

Keywords:

Drug resistance. Environmental monitoring. Cross infection. Air quality

Abstract

Hospital infections (HI) are a serious public health problem in many countries. Several studies have identified strains correlating to surgical site infections, many with multi-resistance. The goals of this study was to quantify, to identify and to verify the resistance profile of microorganisms collected at two hospitals settings, and to alert health professionals how environmental contamination can influence hospital infection rates. For air sampling in operating rooms, intensive care unit and materials sterilization center, the impaction method (Spin Air, IUL®) and passive sedimentation were used. For the isolation of bacteria on surfaces and uniforms contact plates (RODAC®) were used. Identification of the microorganisms was performed using Vitek® 2 Systems. The antibiograms were conducted according to the disk diffusion method recommended by CLSI. The surgical center of hospital B presented more than 500 CFU/m3 in aerial microbial load. In the aerial microbiota of the sampled areas of both hospitals, M. luteus, S. haemolyticus and S. hominis spp hominis were the prevalent microorganisms, with a percentage greater than 30%. On the surfaces and uniforms there was a prevalence of M. luteus (40%) and S. hominis spp hominis (20%) among others, and some of the resistant strains were isolated from environments with microbial load within the recommended limits.

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References

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Potentially pathogenic bacteria isolated from neglected air and surfaces in hospitals

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