A novel approach to Cestrum intermedium (mata-boi)
anatomical and physical-chemical characterization, in vitro biological activities, and metabolites of a Brazilian native species
DOI:
https://doi.org/10.1590/s2175-97902022e20149Keywords:
Scanning electron microscopy, Chemical markers, Secondary metabolites, Alkaloids, Terpenoids, Staphylococcus epidermidisAbstract
The Brazilian native species Cestrum intermedium, known as mata-boi, induces hepatotoxicity and death when ingested by cattle. While most studies on this species focus on toxicological features, our study is the first to describe the anatomy and in vitro biological activities of Cestrum intermedium. We investigated adult leaves and stems by histochemistry, described their anatomy, performed physical-chemical analysis, determined in vitro antioxidant and antimicrobial activities, and identified secondary metabolites. A few noteworthy anatomical features were the anomocytic stomata on the abaxial surface and the absence of trichomes, in addition to the circular shaped petiole with two projections on the adaxial surface. Histochemical analysis showed chemical markers such as alkaloids, usually reported as toxic, and terpenoids. Potassium nitrate (ATR-FTIR) and lupeol palmitate (NMR) were detected on the crude stem extract. Thermogravimetric and physical-chemical analysis provided fingerprint parameters for the species. Minimal Inhibitory Concentration (MIC) assay revealed that Staphylococcus aureus, Staphylococcus epidermidis, and Candida albicans were weakly inhibited by extract samples. Chloroform and ethyl acetate fractions presented high phenolic content, which resulted in in vitro antioxidant activity. These novel features expand the knowledge about this species, considering that previous studies mainly focused on its toxicity. Our study also provided characteristics that may help in avoiding misidentification between Cestrum members, especially when taxonomic keys cannot be employed, as in the absence of flowers and fruits.
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Ahmad KJ. Cuticular studies with special reference to abnormal stomatal cells in Cestrum. J Indian Bot Soc. 1964;43(1):165-177.
American Society for Testing and Materials (ASTM). ASTM E1363-18: Standard Test Method for Temperature Calibration of Thermomechanical Analyzers. 2018.
Ayres MCC, Brandão MS, Vieira-Junior GM, Menor JCAS, Silva HB, Soares MJS, Chaves MH. Antibacterial activity of useful plants and Chemical constituents of the roots of Copernecia prunifera. Braz J Pharmacog. 2008;18(1):90-97. Available at: https://doi.org/10.1590/S0102-695X2008000100017
» https://doi.org/https://doi.org/10.1590/S0102-695X2008000100017
Berlyn GP, Miksche JP. Botanical microtechnique and cytochemistry. Ames: Iowa State University, 1976.
Bhattacharjee I, Ghosh A, Chandra G. Antimicrobial activity of the essential oil of Cestrum diurnum (L.) (Solanales: Solanaceae). Afr J Biotechnol. 2005;4(4):371-374.
Brand MA, Barnasky RRS, Carvalho CA, Buss R, Waltrick DB, Jacinto RC. Thermogravimetric analysis for characterization of the pellets produced with different forest and agricultural residues. Cien Rural. 2018;48(11):01-10.
Brazil. Agência Brasileira de Vigilância Sanitária (ANVISA). Métodos de Farmacognosia. Farmacopeia Brasileira. Brasília: 6 ed., ANVISA, 2019, p. 311 - 340.
Brazil. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Qualidade da carne: do campo à mesa. Brasília: EMBRAPA, 2020.
Brazil. Ministério da Agricultura, Pecuária e Abastecimento (MAPA). Valor da Produção Agropecuária de 2019 atinge recorde de R$ 630,9 bilhões. 2020. Retrieved from: Retrieved from: https://www.gov.br/agricultura/pt-br/assuntos/noticias/valor-da-producao-agropecuaria-encerra-2019-com-r-630-9-bilhoes Access in:11/08/2020.
Carrier M, Loppinet-Serani A, Denux D, Lasnier JM, Ham-Pichavant F, Cansell F, et al. Thermogravimetric analysis as a new method to determine the lignocellulosic composition of biomass. Biomass Bioenergy. 2011;35(1):298-307.
Chew YL, Chan EW, Tan PL, Lim YY, Goh JK. Assessment of phytochemical content, polyphenolic composition, antioxidant and antibacterial activities of Leguminosae medicinal plants in Peninsular Malaysia. BMC Complementary Altern Med. 2011;11(12):1-10.
Cos P, Vlietink AJ, Berghe DV, Maes L. Anti-infective potential of natural products: how to develop a stronger in vitro ‘proof-of-concept’. J Ethnopharmacol. 2006;106(3):290-302.
Furlan FH, Luciolli J, Borelli V, Faria Junior O, Rabelatto SV, Gava A, et al. Intoxicação por Cestrum intermedium (Solanaceae) em bovinos no Estado de Santa Catarina. Acta Sci Vet. 2008;36(3):281-284.
Gallego DC. Cestrum de Colombia (Solanaceae): estudio taxonómico de las especies de tricomas simples. [Master’s dissertation] Bogota: Universidad Nacional de Colombia, 2011.
Henriques AT, Kerber VA, Moreno PRH. Farmacognosia: da planta ao medicamento. Florianópolis/Porto Alegre: UFSC e UFRGS, 2002.
Jáuregui D, Benítez CE. Anatomía foliar de siete especies de Cestrum L. (Solanaceae) y clave para especies de Venezuela. Acta Cient Venez. 2007;58(3-4):75-83.
Khan MA, Inayat H, Khan H, Saeed M, Khan I, Rahman I. Antimicrobial activities of the whole plant of Cestrum nocturnum against pathogenic microorganisms. Afr J Biotechnol . 2011;5(6):612-616.
Kissmann KG, Groth D. Plantas infestantes e nocivas. 2 ed. São Paulo: BASF, 2000.
Liscovsky IJ, Cosa MT. Anatomia comparativa de hoja y tallo em los representantes de Cestreae G. Don (Solanaceae) de Argentina. Gayana Bot. 2005;62(1):33-43.
Liu XK, LI XR, Qiu MH, Nie RL. Triterpene Constituents from Balanophora indica. Acta Bot Yuannanica. 1998;20(3):369-373.
Mensor LL, Menezes FS, Leitão GG, Reis AS, Santos TC, Coube CS, et al. Screening of brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother Res. 2001;15(2):127-130.
Metcalfe CR, Chalk L. Anatomy of the dicotyledons. Oxford: Clarendon Press, 1, 1950.
Miller FA, Wilkins CH. Infrared Spectra and Characteristic Frequencies of Inorganic Ions. An Chem. 1952;24(8):1253-1294.
National Center for Biotechnology Information (NCBI). PubChem Database. Potassium nitrate, CID=24434. 2020. Retrieved from Retrieved from https://pubchem.ncbi.nlm.nih.gov/compound/24434 Access in: 11/08/2020.
» https://pubchem.ncbi.nlm.nih.gov/compound/24434
Nishtha R, Rao A. Phytochemical analysis and anatomical study of two species of Cestrum from Chandigarh. Int J Pharm Sci Res. 2017;8(12):5234-5240.
Poletto M, Dettenborn J, Pistor V, Zeni M, Zattera AJ. Materials produced from plant biomass. Part I: evaluation of thermal stability and pyrolysis of wood. Mater Res. 2010;13(3):375-379.
Prasad MP, Prabhu A, Thakur MS, Yogesh RM. Phytochemical screening, antioxidant potential and antimicrobial activities in three species of cestrum plants. Int J Pharmacol Biol Sci. 2013;4(2):(B)673-678.
Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a Phosphomolybdenum Complex: specific application to the determination of vitamin E. Anal Biochem. 1999;269(2):337-341.
Potassium Nitrate Association (PNA). About potassium nitrate. 2019. Retrieved from http://www.kno3.org
REFLORA. Cestrum L. accepted names in Brazil. Available at: http://floradobrasil.jbrj.gov.br/reflora/listaBrasil/ConsultaPublicaUC/BemVindoConsultaPublicaConsultar.do?invalidatePageControlCounter=1&idsFilhosAlgas=%5B2%5D&idsFilhosFungos=%5B1%2C10%2C11%5D&lingua=en&grupo=5&familia=null&genero=cestrum&especie=&autor=&nomeVernaculo=&nomeCompleto=&formaVida=null&substrato=null&ocorreBrasil=SIM&ocorrencia=OCORRE&endemismo=TODOS&origem=TODOS®iao=QUALQUER&estado=QUALQUER&ilhaOceanica=32767&domFitogeograficos=QUALQUER&bacia=QUALQUER&vegetacao=TODOS&mostrarAte=SUBESP_VAR&opcoesBusca=NOME_ACEITO&loginUsuario=Visitante&senhaUsuario=&contexto=consulta-publica >. Access in: 11/08/2020.
Rojas R, Bustamante B, Bauer J, Fernández I, Albán J, Lock O. Antimicrobial activity of selected Peruvian medicinal plants. J Ethnopharmacol . 2003;88(2-3):199-204.
Silva ATM, Magalhães CG, Duarte LP, Mussel WN, Ruiz ALTG, Shiozawa L, et al. Lupeol and its esters: NMR, powder XRD data and in vitro evaluation of cancer cell growth. Braz J Pharm Sci. 2017;53(3):1-10.
Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol. 1999;29:152-178.
Soares ELC. Estudos taxonômicos em Solanaceae lenhosas no Rio Grande do Sul, Brasil. [Master’s dissertation] Porto Alegre: UFRGS, 2006.
Szabo EM, Homem ICM, Miguel MD, Miguel OG. Determinação de parâmetros físico-químicos e ensaio sistemático fitoquímico preliminar de Cestrum intermedium Sendtn. (Solanaceae). Visao Acad. 2014;15(4):5-16.
United States Department of Agriculture (USDA). Foreign Agricultural Service. Livestock and Poultry: World Markets and Trade. Washington, DC: 2019.
United States Pharmacopeial Convention (USP). <561> Articles of Botanical Origin, USP 40 - NF 35, p. 426-440. Rockville: United States Pharmacopeial Convention. 2017.
Vaz NP. Alcalóides Esteroidais dos Frutos Maduros de Solanum caavurana Vell. [Masters’ in Chemistry dissertation] Curitiba: UFPR. 2008.
Veiga A, Toledo MGT, Rossa LS, Mengarda M, Stofella NCF, Oliveira LJ, et al. Colorimetric Microdillution Assay: Validation of a Standard Method for Determination of MIC, IC50 and IC90 of antimicrobial compounds. J Microbiol Methods. 2019;162:50-61.
Vignoli-Silva M. O Gênero Cestrum L. (Solanaceae) no Brasil extra-amazônico. [thesis] Porto Alegre: UFRGS, 2009.
Wouters ATB, Boabaid FM, Watanabe TTN, Bandarra PM, Correa GLF, Wouters F, et al. Intoxicação espontânea por Cestrum intermedium em bovinos no Sudoeste do Estado do Paraná. Pesq Vet Bras. 2013; 33(1):47-51.
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