Curcuma longa extract protects against 5-fluorouracil-induced oral mucositis in hamsters
DOI:
https://doi.org/10.1590/s2175-97902022000Xe20114Keywords:
5-fluorouracil, Oral mucositis, Curcumin, Oxidative stress, InflammationAbstract
Curcumin, contained at Turmeric (Curcumalonga), can exert many beneficial pleiotropic activities in the gastrointestinal tract. This study evaluated the antioxidant and anti-inflammatory activity of C. longa on 5-fluorouracil (5-FU)-induced oral mucositis (OM) in hamsters. Phytochemical analysis of crude C. longa extract (CLE) was performed to detect the presence of curcumin by TLC and HPLC. Golden Syrian hamsters were orally pre-treated with CLE (5, 50, or 100mg/kg). Cheek pouch samples were subjected to macroscopic and histopathological evaluation. ELISA was performed to quantify the inflammatory cytokines IL-1β and TNF-α. Superoxide dismutase (SOD), glutathione (GSH) and malondialdehyde (MDA) levels were assessed by ultraviolet-visible spectroscopy analysis. Behavior analysis was conducted by the open field test. Curcumin content in the CLE was 0.55%m/m ± 0.0161 (2.84%). The group treated with 5mg/kg CLE showed healing evidence with macroscopic absence of ulceration (p<0.05) and microscopic aspect of re-epithelialization, discrete inflammatory infiltrate and absence of edema. Treatment with 5mg/kg CLE significantly increased GSH levels, and reduced MDA levels and SOD activity (p˂0.05), and decreased IL-1β (p˂0.05) and TNF-α (p˂0.01) levels. A significant reduction in walking distance, ambulation, speed, and rearing was observed for motor activity. Curcumin reduced oxidative stress, inflammation, and motor activity in hamsters with 5-FU-induced OM.
Downloads
References
Aggarwal BB, Harikumar KB. Potential therapeutic effects of curcumin, the anti-inflammatory agent, against neurodegenerative, cardiovascular, pulmonary, metabolic, autoimmune and neoplastic diseases. Int J Biochem Cell Biol. 2009;41(1):40-59.
Ak T, Gulcin I. Antioxidant and radical scavenging properties of curcumin. Chem Biol Interact. 2008;174(1):27-37.
Al-Ansari S, Zecha JA, Barasch A, de Lange J, Rozema FR, Raber-Durlacher JE. Oral Mucositis Induced By Anticancer Therapies. Curr Oral Health Rep. 2015;2(4):202-11.
Bastos CCC, Avila PHM, Filho E, Avila RI, Batista AC, Fonseca SG, et al. Use of Bidens pilosa L. (Asteraceae) and Curcuma longa L. (Zingiberaceae) to treat intestinal mucositis in mice: Toxico-pharmacological evaluations. Toxicol Rep. 2016;3:279-87.
Boussios S, Pentheroudakis G, Katsanos K, Pavlidis N. Systemic treatment-induced gastrointestinal toxicity: incidence, clinical presentation and management. Ann Gastroenterol. 2012;25(2):106-18.
Charantimath S. Use of Curcumin in Radiochemotherapy Induced Oral Mucositis Patients: A Control Trial Study. International Journal of Medical and Health Sciences. 2019;10(3):147-152.
De Souza GE, Ferreira SH. Blockade by antimacrophage serum of the migration of PMN neutrophils into the inflamed peritoneal cavity. Agents Actions. 1985;17(1):97-103.
Delavarian Z, Pakfetrat A, Ghazi A, Jaafari MR, Homaei Shandiz F, Dalirsani Z, et al. Oral administration of nanomicelle curcumin in the prevention of radiotherapy-induced mucositis in head and neck cancers. Spec Care Dentist. 2019;39(2):166-72.
Dos Santos Filho EX, Avila PHM, Bastos CCC, Batista AC, Naves LN, Marreto RN, et al. Curcuminoids from Curcuma longa L. reduced intestinal mucositis induced by 5-fluorouracil in mice: Bioadhesive, proliferative, anti-inflammatory and antioxidant effects. Toxicol Rep . 2016;3:55-62.
Dos Santos Filho EX, da Silva ACG, de Avila RI, Batista AC, Marreto RN, Lima EM, et al. Chemopreventive effects of FITOPROT against 5-fluorouracil-induced toxicity in HaCaT cells. Life Sci. 2018;193:300-8.
Funk JL, Oyarzo JN, Frye JB, Chen G, Lantz RC, Jolad SD, et al. Turmeric extracts containing curcuminoids prevent experimental rheumatoid arthritis. J Nat Prod. 2006;69(3):351-5.
Gao S, Zhou J, Liu N, Wang L, Gao Q, Wu Y, et al. Curcumin induces M2 macrophage polarization by secretion IL-4 and/ or IL-13. J Mol Cell Cardiol. 2015;85:131-9.
Jagetia GC, Rajanikant GK. Role of curcumin, a naturally occurring phenolic compound of turmeric in accelerating the repair of excision wound, in mice whole-body exposed to various doses of gamma-radiation. J Surg Res. 2004;120(1):127-38.
Jaruga E, Salvioli S, Dobrucki J, Chrul S, Bandorowicz-Pikula J, Sikora E, et al. Apoptosis-like, reversible changes in plasma membrane asymmetry and permeability, and transient modifications in mitochondrial membrane potential induced by curcumin in rat thymocytes. FEBS Letters. 1998;433(3):287-93.
Kesarwala AH, Krishna MC, Mitchell JB. Oxidative stress in oral diseases. Oral Dis. 2016;22(1):9-18.
Kwiecien S, Magierowski M. Curcumin: A potent protectant against esophageal and gastric disorders. Int J Mol Sci. 2019;20(6):1477.
Kwon Y. Mechanism-based management for mucositis: option for treating side effects without compromising the efficacy of cancer therapy. OncoTargets Ther. 2016;9:2007-16.
Land Bvt, Blijlevens NMA, Marteijn J, Timal S, Donnelly JP, Witte TJMd, et al. Role of curcumin and the inhibition of NF-κ B in the onset of chemotherapy-induced mucosal barrier injury. Leukemia. 2003;18(2):276-84.
Leão JC, Gomes VB, Porter S. Ulcerative lesions of the mouth: an update for the general medical practitioner. Clinics (São Paulo). 2007;62(6):769-80.
Lee B, Lee H. Systemic administration of curcumin affect anxiety-related behaviors in a rat model of posttraumatic stress disorder via activation of serotonergic systems. Evid Based Complement Alternat Med. 2018;2018:9041309.
Lim YS, Kwon SK, Park JH, Cho CG, Park SW, Kim WK. Enhanced mucosal healing with curcumin in animal oral ulcer model. Laryngoscope. 2016;126(2):E68-73.
Luer S, Troller R, Aebi C. Antibacterial and antiinflammatory kinetics of curcumin as a potential antimucositis agent in cancer patients. Nutr Cancer. 2012;64(7):975-81.
Luer S, Troller R, Jetter M, Spaniol V, Aebi C. Topical curcumin can inhibit deleterious effects of upper respiratory tract bacteria on human oropharyngeal cells in vitro: potential role for patients with cancer therapy induced mucositis? Support Care Cancer. 2011;19(6):799-806.
Normando AGC, de Meneses AG, de Toledo IP, Borges GA, de Lima CL, Dos Reis PED, et al. Effects of turmeric and curcumin on oral mucositis: A systematic review. Phytother Res. 2019;33(5):1318-29.
Panchatcharam M, Miriyala S, Gayathri VS, Suguna L. Curcumin improves wound healing by modulating collagen and decreasing reactive oxygen species. Mol Cell Biochem. 2006;290(1-2):87-96.
Panghal M, Kaushal V, Kadayan S, Yadav JP. Incidence and risk factors for infection in oral cancer patients undergoing different treatments protocols. BMC Oral Health. 2012;12(1):1-12.
Patil K, Guledgud MV, Kulkarni PK, Keshari D, Tayal S. Use of curcumin mouthrinse in radio-chemotherapy induced oral mucositis patients: A pilot study. J Clin Diagn Res. 2015;9(8):59-62.
Pisoschi AM, Pop A. The role of antioxidants in the chemistry of oxidative stress: A review. Eur J Med Chem. 2015;97:55-74.
Rao S, Dinkar C, Vaishnav LK, Rao P, Rai MP, Fayad R, et al. The Indian spice turmeric delays and mitigates radiation-induced oral mucositis in patients undergoing treatment for head and neck cancer: An investigational study. Integrative cancer therapies. 2014;13(3):201-10.
Reddy AC, Lokesh BR. Effect of dietary turmeric (Curcuma longa) on iron-induced lipid peroxidation in the rat liver. Food Chem Toxicol. 1994;32(3):279-83.
Schmidt TR, Curra M, Wagner VP, Martins MAT, Oliveira AC, Batista AC. Mucoadhesive formulation containing Curcuma longa L. reduces oral mucositis induced by 5-fluorouracil in hamsters. 2019,33(4):881-890.
Completar referencia
Shirane HY, Oda DY, Pinheiro TC, Cunha MRd. Implantes de biomateriais em falha óssea produzida na fíbula de ratos. Rev Bras Ortop. 2010;45(5):478-82.
Siviero A, Gallo E, Maggini V, Gori L, Mugelli A, Firenzuoli F, et al. Curcumin, a golden spice with a low bioavailability. J Herbal Med. 2015;5(2):57-70.
Sonis ST. New thoughts on the initiation of mucositis. Oral Diseases. 2010;16(7):597-600.
Sreejayan, Rao MN. Curcuminoids as potent inhibitors of lipid peroxidation. J Pharm Pharmacolo. 1994;46(12):1013-6.
Stanic Z. Curcumin, a compound from natural sources, a true scientific challenge - A review. Plant Foods Hum Nutr. 2017;72(1):1-12.
Tawfik SS, Abouelella AM, Shahein YE. Curcumin protection activities against gamma-rays-induced molecular and biochemical lesions. BMC Res Notes. 2013;6:375.
Willenbacher E, Khan SZ, Mujica SCA, Trapani D, Hussain S, Wolf D, et al. Curcumin: New insights into an ancient ingredient against cancer. Int J Mol Sci . 2019;20(8):1808.
Yin H, Guo Q, Li X, Tang T, Li C, Wang H, et al. Curcumin suppresses IL-1beta secretion and prevents inflammation through inhibition of the NLRP3 inflammasome. J Immunol. 2018;200(8):2835-46.
Downloads
Published
Issue
Section
License
Copyright (c) 2022 Brazilian Journal of Pharmaceutical Sciences
This work is licensed under a Creative Commons Attribution 4.0 International License.
All content of the journal, except where identified, is licensed under a Creative Commons attribution-type BY.
The on line journal has open and free access.
How to Cite
Funding data
-
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Grant numbers 001
