Chemical characterization, cytotoxicity, antimicrobial and antioxidant potential of Justicia pectoralis Jacq and Croton jacobinensis Baill extracts


  • Leilanne Márcia Nogueira de Oliveira Northeast Biotechnology Center, Federal University of Ceará, Fortaleza, Brazil
  • Maria do Socorro Rocha Bastos Embrapa Agroindustria Tropical, Fortaleza, Brazil
  • Ana Cristina Silva de Lima Northeast Biotechnology Center, Federal University of Ceará, Fortaleza, Brazil
  • Evânia Altina Teixera de Figueiredo Department of Food Engineering, Federal University of Ceará, Fortaleza, Brazil
  • Flayanna Gouveia Braga Dias Department of Food Engineering, Federal University of Ceará, Fortaleza, Brazil
  • Larissa Morais Ribeiro da Silva Department of Food Engineering, Federal University of Ceará, Fortaleza, Brazil
  • Selene Dahia Benevides Embrapa Agroindustria Tropical, Fortaleza, Brazil
  • Paulo Riceli Vasconcelos Ribeiro Embrapa Agroindustria Tropical, Fortaleza, Brazil
  • Luiz Bruno de Sousa Sabino * Department of Chemical Engineering, Federal University of Ceará, Fortaleza, Brazil



Natural extracts; Bioactive compounds; Identification; Therapeutics


In this research, aqueous and ethanolic extracts from Justicia pectoralis Jacq and Croton Jacobinensis Baill were characterized. The UPLC-QTOF-MSE analysis was performed on the extracts identified, predominantly, flavonoids, tannins and acids. The extracts did not indicate toxicity in human epithelial cells. Cjacobinensis presented a concentration of phenolics 60.5% higher than Jpectoralis in all scenarios evaluated and, for both samples, the hydroalcoholic extract at 70% exhibited the best efficiency in the extraction (14501.3 and 32521.5 mg GAE 100 g-1 for Jpectoralis and Cjacobinensis, respectively). The antioxidant activity presented a positive correlation with the concentration of phenolics, being 1.186,1 and 1.507,9 μM of Trolox for Jpectoralis and Cjacobinensis at 70% of ethanol; however, it was not verified statistical difference between the ethanolic solutions (p < 0.05). The antimicrobial activity of Jpectoralis extracts was highlighted once was the most effective against gram-positive bacteria. The results suggest that both Jpectoralis and Cjacobinensis extracts present the potential to be applied as natural additives due to their antioxidant and antimicrobial activity and safety. Thus, it is suggesting the development of studies that could investigate the interaction of these plant extracts with food matrices is required.


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Agra MF, Freitas PF de, Barbosa-Filho JM. Synopsis of the plants known as medicinal and poisonous in Northeast of Brazil. Rev Bras Farmacogn. 2007;17(1):114-40.

Artega JJM, Perea EM, Váron EY, Ospina WFS, Osório JN, Martínez OMM. Climate influence on chemical composition and antioxidant activity of Justicia pectoralis Jacq. Rev Cuba de Farm. 2011;45(1):88-100.

Baqueiro-Peña I, Guerrero-Beltrán JÁ. Physicochemical and antioxidant characterization of Justicia spicigera. Food Chem. 2017;218:305-12.

Brandt AL, Castillo A, Harris KB, Keeton JT, Hardin MD, Taylor TM. Inhibition of Listeria monocytogenes by Food Antimicrobials Applied Singly and in Combination. J Food Sci. 2010;75(9):M557.

Branen JK, Davidson PM. Enhancement of nisin, lysozyme, and monolaurin antimicrobial activities by ethylenediaminetetraacetic acid and lactoferrin. Int J Food Microbiol. 2004;90(1):63-74.

Brighenti FL, Salvador MJ, Delbem ACB, Delbem ÁCB, Oliveira MAC, Soares CP, et al. Systematic screening of plant extracts from the brazilian pantanal with antimicrobial activity against bacteria with cariogenic relevance. Caries Res. 2014;48(5):353-60.

Calo JR, Crandall PG, O’Bryan CA, Ricke SC. Essential oils as antimicrobials in food systems - A review. Food Control. 2015;54:111-9.

Corrêa GM, Alcântara AF de C. Chemical constituents and biological activities of species of Justicia: a review. Rev Bras Farmacogn . 2012;22(1):220-38.

Chanfrau JER, Hernández ODL, Figueredo YN, Ferrada CR, Mendoza AN. obtention of dry extract from aqueous extracts of Justicia pectoralis jacq. (tilo). Rev Cuban Plan Med. 2013;18(4):543-554.

da Silva AFG, Feitosa BH, Lima VT, Silva VFB. Antioxidant activity and total phenol, flavonoid and tannin contents of Croton floribundus and Croton urucurana. Enc Bio. 2019;16(30):11-22.

da Silva Port’s P, Chisté RC, Godoy HT, Prado MA. The phenolic compounds and the antioxidant potential of infusion of herbs from the Brazilian Amazonian region. Food Res Int. 2013;53(2):875-81.

Dall’Acqua S, Sinan KI, Sut S, Ferrarese I, Etienne OK, Mahomoodally MF, et al. Evaluation of antioxidant and enzyme inhibition properties of Croton hirtus L’Hér. extracts obtained with different solvents. Molecules. 2021;26(7):1902.

Dutta S, Dey P, Chaudhuri TK. Assessment of bioactive phytochemicals present in the root of Croton bonplandianum available in the sub-Himalayan region of West Bengal. Int J Pharm Pharm Sci. 2013;5(Suppl 4):540-5.

Feng C, García-Martín JF, Lavado MB, López-Barrera M del C, Álvarez-Mateos P. Evaluation of different solvents on flavonoids extraction efficiency from sweet oranges and ripe and immature Seville oranges. Int J Food Sci Technol. 2020;55(9):3123-34.

Furtado JM, Amorim Á da S, Fernandes MV de M, Oliveira MAS. Atividade antimicrobiana do extrato aquoso de Eucalyptus globulus, Justicia pectoralis e Cymbopogon citratus frente a bactérias de interesse. J Heal Sci. 2015;17(4):233.

Geris R, Silva IG da, Silva HHG da, Barison A, Rodrigues-Filho E, Ferreira AG. Diterpenes of Copaifera reticulata Ducke with larvicidal activity against Aedes aegypti (L.) (Diptera, Culicidae). Rev Inst Med Trop São Paulo. 2008;50(1):26-28.

Guimarães TLF, da Silva LMR, Lima CB, Magalhães FEA, de Figueiredo EAT. Antimicrobial activity of microcapsules with aqueous extract of chambá (Justicia pectoralis Jacq). Rev Ciênc Agronon. 2020;51(2):e20186471.

Kalyne L, Moreira A, Silva AH, Socorro G, Viana DB. Justicia pectoralis, a coumarin medicinal plant have potential for the development of antiasthmatic drugs ? Rev Bras Farmacogn . 2017;27(6):794-802.

Keerthana G, Kalaivani MK, Sumathy A. In-vitro alpha amylase inhibitory and anti-oxidant activities of ethanolic leaf extract of Croton bonplandianum. Asian J Pharm Clin Res. 2013;6(4):32-6.

Kulkarni RR, Shurpali K, Puranik VG, Sarkar D, Joshi SP. Antimycobacterial labdane diterpenes from Leucas stelligera. J Nat Prod. 2013;76(10):1836-41.

Larrauri JA, Rupérez P, Saura-Calixto F. Effect of drying temperature on the stability of polyphenols and antioxidant activity of red grape pomace peels. J Agric Food Chem 1997;45(4):1390-3.

Li J, Wang X, Wang Y, Lu C, Zheng D, Zhang J. Isoquercitrin, a flavonoid glucoside, exerts a positive effect on osteogenesis in vitro and in vivo. Chem Biol Interact. 2019;297:85-94.

Liu CP, Xu JB, Zhao JX, Xu CH, Dong L, Ding J, et al. Diterpenoids from Croton laui and Their Cytotoxic and Antimicrobial Activities. J Nat Prod . 2014; 77(4):1013-20.

Lizcano LJ, Bakkali F, Begoña Ruiz-Larrea M, Ruiz-Sanz JI. Antioxidant activity and polyphenol content of aqueous extracts from Colombian Amazonian plants with medicinal use. Food Chem . 2010;119(4):1566-1570.

Lv F, Liang H, Yuan Q, Li C. In vitro antimicrobial effects and mechanism of action of selected plant essential oil combinations against four food-related microorganisms. Food Res Int . 2011;44(9):3057-3064.

Ma X, Moilanen J, Laaksonen O, Yang W, Tenhu E, Yang B. Phenolic compounds and antioxidant activities of tea-type infusions processed from sea buckthorn (Hippophaë rhamnoides) leaves. Food Chem . 2019;272:1-11.

Mak YW, Chuah LO, Ahmad R, Bhat R. Antioxidant and antibacterial activities of hibiscus (Hibiscus rosa-sinensis L) and Cassia (Senna bicapsularis L.) flower extracts. J King Saud Univ Sci. 2013;25(4):275-282.

Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65(1-2):55-63.

Moosavi F, Hosseini R, Rajaian H, Silva T, Magalhães DES, Saso L, et al. Derivatives of caffeic acid, a natural antioxidant, as the basis for the discovery of novel nonpeptidic neurotrophic agents. Bioorg Med Chem. 2017;25(12):3235-3246.

Neves IA, Camara CA. Acaricidal activity against Tetranychus urticae and essential oil composition of four Croton species from Caatinga biome in northeastern Brazil. Nat Prod Commun. 2011;6(6):893-899.

Nunes TFS, Cordeiro MF, Beserra FG, Souza ML, da Silva WAV, Ferreira MRA, et al. Organic extract of Justicia pectoralis Jacq. leaf inhibits interferon-γ secretion and has bacteriostatic activity against Acinetobacter baumannii and Klebsiella pneumoniae. Eve Based Complement Alternat Med. 2018;2018:5762368.

Pontes WT, Oliveira JC, Da Câmara CA, De Assis CP, De Oliveira JV, Júnior MGG, et al. Effects of the ethanol extracts of leaves and branches from four species of the genus Croton on Tetranychus urticae Koch (Acari: Tetranychidae). BioAssay. 2011;6:3-14.

Rameshkumar A, Sivasudha T. In vitro antioxidant and antibacterial activity of aqueous and methanolic extract of Mollugo nudicaulis Lam. leaves. Asian Pac J Trop Med. 2012;2(2):5825-5900.

Ramos SCS, Oliveria JCS, Câmara ACG, Castelar I, Carvalho AFFU, Lima-Filho JV. Antibacterial and cytotoxic properties of some plant crude extracts used in Northeastern folk medicine. Rev Bras Farmacogn . 2009;19(2A):376-381.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 1999;26(9-10):1231-1237.

Rufino MSM, Alves RE, Brito ES, Pérez-Jimenez J, Saura-Calixto F, Mancini-Filho J. Bioactive compounds and antioxidant capacities of 18 non-traditional tropical fruits from Brazil. Food Chem . 2010;121:996-1002.

Ryan EM, Duryee MJ, Hollins A, Dover SK, Pirruccello S, Sayles H, et al. Antioxidant properties of citric acid interfere with the uricase-based measurement of circulating uric acid. J Pharm Biomed Anal. 2019;164:460-466.

Sá Firmino NC, Alexandred FSO, Vasconcelos MA, Pinheiro AA, Arruda FVS, Guedes MLS, et al. Diterpenes isolated from Croton blanchetianus Baill: Potential compounds in prevention and control of the oral Streptococci biofilms. Ind Crops Prod. 2019;131:371-377.

Silva CG, Raulino RJ, Cerqueira DM, Mannarino SC, Pereira MD, Panek AD, et al. In vitro and in vivo determination of antioxidant activity and mode of action of isoquercitrin and Hyptis fasciculata Phytomedicine. 2009;18(8):761-767.

Singh B, Dutt N, Kumar D, Singh S, Mahajan R. Taxonomy, ethnobotany and antimicrobial activity of Croton bonplandianum, Euphorbia hirta and Phyllanthus fraternus Int J Dev Res. 2011;2(1):21-29.

Suresh Reddy Y, Chinnala KM, Nath KVS, Elsani MM, Preethi B, Boggula N. In vitro evaluation of antioxidant activity of different extracts of Justicia gendarussa leaf. Pharma Chem. 2015;7(8):21-24.

Tang Y, Lou F, Wang J, Li Y, Zhuang S. Coumaroyl flavonol glycosides from the leaves of Ginkgo biloba. Phytochem. 2001;58(8):1251-1256.

Viegas Júnior C. Terpenos com atividade inseticida: uma alternativa para o controle químico de insetos. Quim Nova. 2003;26(3):390-400.

Villamizar J, Pittelaud JP, Rodrigues JR, Gamboa N, Canudas N, Tropper E, et al. Synthesis and antimalarial activities of optically active labdane-type diterpenes. Nat Prod Res. 2009;23(10):891-902.

Wang Y, Tang C, Zhang H. Hepatoprotective effects of kaempferol 3-O-rutinoside and kaempferol 3-O-glucoside from Carthamus tinctorius L. on CCl4-induced oxidative liver injury in mice. J Food Drug Anal. 2015;23(2):310-317.

Xu W, Liu W, Liang Q. Chemical constituents from Croton species and their biological activities. Molecules. 2018;23(9):2333-2370.

Yun J, Woo ER, Lee DG. Effect of isoquercitrin on membrane dynamics and apoptosis-like death in Escherichia coli Biochim Biophys Acta Biomembr. 2018;1860(2):357-363.






Original Article

How to Cite

Chemical characterization, cytotoxicity, antimicrobial and antioxidant potential of Justicia pectoralis Jacq and Croton jacobinensis Baill extracts. (2023). Brazilian Journal of Pharmaceutical Sciences, 59, e21224.