Biological screening of herbal extracts and essential oil from Plectranthus species

α-amylase and 5-lipoxygenase inhibition and antioxidant and anti-Candida potentials


  • Simony Carvalho Mendonça Laboratory of Phytochemistry and Medicinal Plants, Federal University of Lavras, MG, Brazil
  • Smail Aazza Laboratory of Phytochemistry, National Agency of Medicinal and Aromatic Plants, BP 159, Taounate, Morocco
  • Alexandre Alves de Carvalho Laboratory of Phytochemistry and Medicinal Plants, Federal University of Lavras, MG, Brazil
  • Diogo Mendes da Silva Laboratory of Phytochemistry and Medicinal Plants, Federal University of Lavras, MG, Brazil
  • Nelma de Mello Silva Oliveira Laboratory of Microbiology, University of Alfenas (UNIFENAS), MG, Brazil SCImago image
  • José Eduardo Brasil Pinto Pinto Laboratory of Phytochemistry and Medicinal Plants, Federal University of Lavras, MG, Brazil
  • Suzan Kelly Vilela Bertolucci Laboratory of Phytochemistry and Medicinal Plants, Federal University of Lavras, MG, Brazil



Plectranthus amboinicus; Plectranthus barbatus; Plectranthus ornatus; Extraction methods; Biological activities


The phenolic compound content, the antioxidant and α-amylase inhibition potentials of different extracts of the Plectranthus amboinicusP. barbatus and P. ornatus were evaluated. We also evaluated the influence of plant growth and harvest time on the chemical composition of the essential oil (EO) of P. amboinicus, its antioxidant and anti-Candida activities and the α-amylase and lipoxygenase inhibitions. The turbo-extract of P. barbatus showed the greatest phenolic compound content and antioxidant activity. No α-amylase inhibition activity was observed in the analyzed extracts, but the turbo-extraction and refluxing extracts possessed high antioxidant activities. Protected cultivation and morning harvest conditions gave the best antioxidant activities, which was associated to the highest carvacrol content. P. amboinicus EO antioxidant activity could contribute to the reduction of oxidative stress in diabetes. Causal Candida strains of diabetic foot ulcers showed sensitivity to P. amboinicus EO. C. albicans and C. dubliniensis were the most sensitive of the selected Candida strains. Turbo-extracts or refluxing of the three species extracts and the EO of P. amboinicus should be considered as a potential candidate for the management the complications of type 2 diabetes.


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Aazza S, Lyoussi B, Megias C, Cortes-Giraldo I, Vioque J, Figueiredo AC, et al. Anti-oxidant, anti-inflammatory and anti-proliferative activities of Moroccan commercial essential oils. Nat Prod Commun. 2014;9(4):587-594.

Ahn M-R, Kumazawa S, Usui Y, Nakamura J, Matsuka M, Zhu F, et al. Antioxidant activity and constituents of propolis collected in various areas of China. Food Chem. 2007;101(4):1383-1392.

Alasbahi RH, Melzig MF. Plectranthus barbatus: a review of phytochemistry, ethnobotanical uses and pharmacology - Part 1. Planta Med. 2010;76(7):653-61.

Arumugam G, Swamy MK, Sinniah UR. Plectranthus amboinicus (Lour.) Spreng: botanical, phytochemical, pharmacological and nutritional significance. Molecules. 2016;21(4):369.

Bandeira JM, Barbosa FF, Barbosa LMP, Rodrigues ICS, Bacarin MA, Peters JA, et al. Composição do óleo essencial de quatro espécies do gênero Plectranthus. Rev Bras Plantas Medic. 2011;13(2):157-164.

Bayramoglu G, Senturk H, Bayramoglu A, Uyanoglu M, Colak S, Ozmen A, et al. Carvacrol partially reverses symptoms of diabetes in STZ-induced diabetic rats. Cytotechnology. 2014;66(2):251-257.

Bezerra RdCdF, Neto FBdO, Silva FFMd, Bertini LM, Alves LA. Seasonal effect in essential oil composition and antioxidant activity of Plectranthus amboinicus leaves. Biosci J. 2017;33(6):1608-1616.

Bibiano CS, de Carvalho AA, Bertolucci SKV, Torres SS, Corrêa RM, Pinto JEBP. Organic manure sources play fundamental roles in growth and quali-quantitative production of essential oil from Dysphania ambrosioides L. Ind Crop Prod. 2019;139:111512.

Boulanouar B, Abdelaziz G, Aazza S, Gago C, Miguel MG. Antioxidant activities of eight Algerian plant extracts and two essential oils. Ind Crop Prod . 2013;46:85-96.

Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. Food Sci Technol. 1995;28(1):25-30.

Agência Nacional de Vigilância Sanitária (Brasil). Resolução nº. 49, de 23 de novembro de 2010. Aprova a Farmacopeia Brasileira, 5ª edição e dá outras providências. Diário Oficial da União 24 nov 2010; Seção 1.

Brito E, Gomes E, Falé PL, Borges C, Pacheco R, Teixeira V, et al. Bioactivities of decoctions from Plectranthus species related to their traditional use on the treatment of digestive problems and alcohol intoxication. J Ethnopharmacol. 2018;220:147-154.

Chizzola R, Michitsch H, Franz C. Antioxidative properties of Thymus vulgaris leaves: comparison of different extracts and essential oil chemotypes. J Agric Food Chem . 2008;56(16):6897-6904.

Cid-Perez TS, Avila-Sosa R, Ochoa-Velasco CE, Rivera-Chavira BE, Nevarez-Moorillon GV. Antioxidant and antimicrobial activity of mexican oregano (Poliomintha longiflora) essential oil, hydrosol and extracts from waste solid residues. Plants. 2019;8(1):1-13.

CLSI Clinical and Laboratory Standards Institute -Method for antifungal disk diffusion susceptibility testing of yeasts (Document M44-A2). In: 2nd ed Wayne; 2009.

Dhakshinya M, Vishnu PV, Gayathri R, Sundaram R. In vitro α-amylase and α-glucosidase inhibitory activity of isolated fraction one from Plectranthus amboinicus. Drug Inven Today. 2019;12(4):788-790.

Dobrian AD, Morris MA, Taylor-Fishwick DA, Holman TR, Imai Y, Mirmira RG, et al. Role of the 12-Lipoxygenase Pathway in Diabetes Pathogenesis and Complications. Pharmacol Ther. 2019:195:100-110.

Domingueti CP, Dusse LMSA, Carvalho MdG, de Sousa LP, Gomes KB, Fernandes AP. Diabetes mellitus: The linkage between oxidative stress, inflammation, hypercoagulability and vascular complications. J Diabetes Complications. 2016;30(4):738-745.

Dong C, Liu S, Cui Y, Guo Q. 12-Lipoxygenase as a key pharmacological target in the pathogenesis of diabetic nephropathy. Eur J Pharmacol. 2020; 879:173122.

El-Hawary S, El-Sofany R, Abdel-Monem A, Ashour R, Sleem A. Seasonal variation in the composition of Plectranthus amboinicus (Lour.) Spreng essential oil and its biological activities. Am J Essent Oils Nat Prod. 2013;1(2):11-18.

Ezhumalai M, Radhiga T, Pugalendi KV. Antihyperglycemic effect of carvacrol in combination with rosiglitazone in high-fat diet-induced type 2 diabetic C57BL/6J mice. Mol Cell Biochem. 2014;385(1):23-31.

Fatima S, Akhtar MF, Ashraf KM, Sharif A, Saleem A, Akhtar B, et al. Antioxidant and alpha amylase inhibitory activities of Fumaria officinalis and its antidiabetic potential against alloxan induced diabetes. Cell Mol Biol (Noisy-le-grand). 2019;65(2):50-57.

Falé P, Borges C, Madeira P, Ascensao L, Araújo ME, Florêncio M, et al. Rosmarinic acid, scutellarein 4′-methyl ether 7-O-glucuronide and (16S)-coleon E are the main compounds responsible for the antiacetylcholinesterase and antioxidant activity in herbal tea of Plectranthus barbatus (falso boldo). Food Chem . 2009;114(3):798-805.

Gülçin İ, Huyut Z, Elmastaş M, Aboul-Enein HY. Radical scavenging and antioxidant activity of tannic acid. Arab J Chem. 2010;3(1):43-53.

Imbert JL, G. Gomez JV, Escudero RB, Blasco JL. Onicomicosis por levaduras no comunes en diabéticos de un centro de salud. SEMERGEN - Med Fam. 2016;42(7):449-457.

Kabouche A, Kabouche Z, Öztürk M, Kolak U, Topçu G. Antioxidant abietane diterpenoids from Salvia barrelieri. Food Chem . 2007;102(4):1281-1287.

Kirakosyan A, Gutierrez E, Ramos Solano B, Seymour EM, Bolling SF. The inhibitory potential of Montmorency tart cherry on key enzymes relevant to type 2 diabetes and cardiovascular disease. Food Chem . 2018;252:142-146.

Koti BC, Gore A, Thippeswamy AH, Swamy AH, Kulkarni R. Alcoholic leaf extract of Plectranthus amboinicus regulates carbohydrate metabolism in alloxan-induced diabetic rats. Indian J Pharmacol. 2011;43(3):286-290.

Kumar D, Banerjee T, Chakravarty J, Singh SK, Dwivedi A, Tilak R. Identification, antifungal resistance profile, in vitro biofilm formation and ultrastructural characteristics of Candida species isolated from diabetic foot patients in Northern India. Indian J Med Microbiol. 2016;34(3):308-314.

Lakshmanan GMA, Manikandan S. Review on pharmacological effects of Plectranthus forskohlii (Willd) Briq”. Int Lett Nat Sci. 2015;28:1-9.

Mesquita LSF, Matos TS, Ávila FDN, Batista ADS, Moura AF, de Moraes MO, et al. Diterpenoids from leaves of cultivated Plectranthus ornatus. Planta Med . 2021;87(1-02):124-135.

Miguel MG. Antioxidant and anti-inflammatory activities of essential oils: a short review. Molecules. 2010;15(12):9252-9287.

Mota L, Figueiredo AC, Pedro LG, Barroso JG, Miguel MG, Faleiro ML, et al. Volatile-oils composition, and bioactivity of the essential oils of Plectranthus barbatus, P. neochilus, and P. ornatus grown in Portugal. Chem Biodivers. 2014;11(5):719-32.

Murthy PS, Ramalakshmi K, Srinivas P. Fungitoxic activity of Indian borage (Plectranthus amboinicus) volatiles. Food Chem . 2009;114(3):1014-1018.

Niveditha VR, Sridhar K. Antioxidant activity of raw, cooked and Rhizopus oligosporus fermented beans of Canavalia of coastal sand dunes of Southwest India. J Food Sci Technol . 2014;51(11):3253-3260.

Oh J, Jo H, Cho AR, Kim S-J, Han J. Antioxidant and antimicrobial activities of various leafy herbal teas. Food Control. 2013;31(2):403-409.

Pereira CA, Corrêa AD, Pereira LLS, Chagas PMB, Santos CD, de Souza SP. Inibição de enzimas digestivas por extratos de pó comercial de Hoodia gordonii utilizado no tratamento da obesidade. Rev Bras Biociências. 2011;9(3):265-269.

Popova M, Bankova V, Butovska D, Petkov V, Nikolova-Damyanova B, Sabatini AG, et al. Validated methods for the quantification of biologically active constituents of poplar-type propolis. Phytochem Anal. 2004;15(4):235-240.

Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem. 1999;269(2):337-341.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999;26(9):1231-1237.

Rodrigues CF, Rodrigues ME, Henriques M. Candida sp. infections in patients with Diabetes Mellitus. J Clin Med. 2019;8(1):76.

Romano CS, Abadi K, Repetto V, Vojnov AA, Moreno S. Synergistic antioxidant and antibacterial activity of rosemary plus butylated derivatives. Food Chem . 2009;115(2):456-461.

Saad B, Zaid H, Shanak S, Kadan S. Prevention and treatment of obesity-related diseases by diet and medicinal plants. In: Springer editor. Anti-diabetes and anti-obesity medicinal plants and phytochemicals. Switzerland Springer. 2017. p. 95-128.

Sharma P, Joshi T, Joshi T, Chandra S, Tamta S. Molecular dynamics simulation for screening phytochemicals as α-amylase inhibitors from medicinal plants. J Biomol Struct Dyn. 2020;39(17):1-15.

Slinkard K, Singleton VL. Total phenol analysis: automation and comparison with manual methods. Am J Enol Vitic. 1977;28(1):49-55.

TGA Guidance on equivalence of herbal extracts in complementary medicines. [citad 2011 Apr 10]. Available from: Available from: complementary-medicines

» complementary-medicines

Vera R, Mondon JM, Pieribattesti JC. Chemical composition of the essential oil and aqueous extract of Plectranthus amboinicus. Planta Med . 1993;59(2):182-183.

Xiao Z, Storms R, Tsang A. A quantitative starch-iodine method for measuring alpha-amylase and glucoamylase activities. Anal Biochem . 2006;351(1):146-148.




How to Cite

Simony Carvalho Mendonça, Smail Aazza, Alexandre Alves de Carvalho, Diogo Mendes da Silva, Nelma de Mello Silva Oliveira, José Eduardo Brasil Pinto Pinto, & Suzan Kelly Vilela Bertolucci. (2023). Biological screening of herbal extracts and essential oil from Plectranthus species: α-amylase and 5-lipoxygenase inhibition and antioxidant and anti-Candida potentials. Brazilian Journal of Pharmaceutical Sciences, 59, e21117.



Original Article