Applicability of an HPLC method for analysis of alcoholic and glycolic Brazilian green-propolis extracts


  • Camila Vecchi Postgraduate Program in Pharmaceutical Sciences, Laboratory of Research and Development of Drug Delivery Systems, Department of Pharmacy, State University of Maringa, Maringa, Parana, Brazil
  • Rafaela Said dos Santos Postgraduate Program in Pharmaceutical Sciences, Laboratory of Research and Development of Drug Delivery Systems, Department of Pharmacy, State University of Maringa, Maringa, Parana, Brazil
  • Marcos Bruschi Postgraduate Program in Pharmaceutical Sciences, Laboratory of Research and Development of Drug Delivery Systems, Department of Pharmacy, State University of Maringa, Maringa, Parana, Brazil



Propolis, High-performance liquid chromatography (HPLC), Glycolic extract, Ethanolic extract, Phytochemicals


Brazilian green propolis has been widely used in food and pharmaceutical products due to its valuable source of phenolic compounds and versatile biological activities. The development and validation of analytical methods are extremely useful for the characterization and quality control of products containing propolis. Therefore, the aim of this study was to optimize, validate and investigate the applicability of a reversed-phase HPLC method for analysis of different types of Brazilian green propolis extracts (glycolic and ethanolic). The method showed to be selective for the propolis phenolic markers. The analysis of variance and residues demonstrated that the method had significant linear regression, without lack of fit. It was also a precise, accurate and robust method, which was of utmost importance to analyze both glycolic and ethanolic extracts and at different concentrations. Moreover, as these products can display most complex matrices to analyze, a valid HPLC method can also prove to be specific and versatile.


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How to Cite

Vecchi, C., Said dos Santos, R., & Bruschi, M. . (2023). Applicability of an HPLC method for analysis of alcoholic and glycolic Brazilian green-propolis extracts. Brazilian Journal of Pharmaceutical Sciences, 59.



Original Article