A fully validated microbiological assay to evaluate the potency of ceftriaxone sodium
| Dublin Core | PKP Metadata Items | Metadata for this Document | |
| 1. | Title | Title of document | A fully validated microbiological assay to evaluate the potency of ceftriaxone sodium |
| 2. | Creator | Author's name, affiliation, country | Maria Luisa Manfio; Franciscano University Center; Pharmacy Course; |
| 2. | Creator | Author's name, affiliation, country | Danielle Araújo Agarrayua; Franciscano University Center; Pharmacy Course; |
| 2. | Creator | Author's name, affiliation, country | Jaison Carlosso Machado; Federal University of Rio Grande do Sul; Faculty of Pharmacy; |
| 2. | Creator | Author's name, affiliation, country | Cleber Alberto Schmidt; Federal University of Bahia; Faculty of Pharmacy; Department of Medicines; |
| 3. | Subject | Discipline(s) | |
| 3. | Subject | Keyword(s) | |
| 4. | Description | Abstract | Ceftriaxone (CFTX) sodium is a third-generation, broad-spectrum cephalosporin that is resistant to beta-lactamases. An alternative bioassay for the assessment of the potency of this drug in pharmaceutical formulations has not been previously reported. Thus, this paper reports the development and full validation of a 3 x 3 agar diffusion bioassay using a cylinder-plate method to quantify CFTX sodium in pharmaceutical samples. The strain Staphylococcus aureus ATCC 6538P was used as the test microorganism, and the results of the proposed bioassay displayed high linearity, precision, accuracy, specificity and robustness. All potency results were statistically analyzed using an analysis of variance (ANOVA) and were found to be linear (r=0.99999) in the range of 16-64 µg/mL, accurate (100.5%), and precise [repeatability: relative standard deviation (RSD)=1.4%; intermediate precision: between-day RSD=2.1% and between-analyst RSD=2.5%]. The specificity of the bioassay was determined by evaluating a degraded sample (50 ºC) at 0, 24 and 48 hours as compared against the results from the pharmacopeial liquid chromatography method for CFTX. The results validated the proposed microbiological assay, which allows reliable quantitation of CFTX in pharmaceutical samples. Moreover, it is a useful, simple and low-cost alternative method for monitoring the quality of this medicine. |
| 5. | Publisher | Organizing agency, location | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
| 6. | Contributor | Sponsor(s) | |
| 7. | Date | (YYYY-MM-DD) | 2013-12-01 |
| 8. | Type | Status & genre | Peer-reviewed Article |
| 8. | Type | Type | |
| 9. | Format | File format | |
| 10. | Identifier | Uniform Resource Identifier | https://www.revistas.usp.br/bjps/article/view/76555 |
| 10. | Identifier | Digital Object Identifier (DOI) | http://dx.doi.org/10.1590/S1984-82502013000400015 |
| 11. | Source | Title; vol., no. (year) | Brazilian Journal of Pharmaceutical Sciences (Impresso); Vol 49, No 4 (2013) |
| 12. | Language | English=en | en |
| 13. | Relation | Supp. Files | |
| 14. | Coverage | Geo-spatial location, chronological period, research sample (gender, age, etc.) | |
| 15. | Rights | Copyright and permissions |
Copyright (c) 2017 Brazilian Journal of Pharmaceutical Sciences (Impresso) This work is licensed under a Creative Commons Attribution 4.0 International License. |