Apoptosis as a prognostic marker in canine mammary tumors by TUNEL

Authors

  • Kariny Sanches Pereira
  • Débora Aparecida Pires de Campos Zuccari Centro Universitário de Rio Preto, Faculdade de Medicina Veterinária, Departamento de Cirurgia e Clínicas, São José do Rio Preto, SP
  • Patrícia Maluf Cury Faculdade de Medicina de Rio Preto, Departamento de Patologia, São José do Rio Preto, SP
  • José Antônio Cordeiro Faculdade de Medicina de Rio Preto, Departamento de Patologia, São José do Rio Preto, SP

DOI:

https://doi.org/10.1590/S1413-95962003000500007

Keywords:

Apoptosis, Prognosis, Breast cancer, Bitch

Abstract

Apoptosis plays an important role in oncogenesis determining tumor growth and aggressiveness. The aim of this study was to examine the relationship between apoptosis, diagnosis and prognosis in canine mammary tumors. Thirty bitches were submitted to tumor extirpation and a method of labeling apoptotic cells, that labels DNA fragments, by terminal deoxynucleotidyl-tranferase (TdT) mediated by 5' deoxy-uridine-triphosphate (dUTP) nick and labeling (TUNEL) was used. The apoptotic cells were counted in ten high power fields (10HPF). Statistical results showed that when there are more than 30 apoptotic cells/10HPF the prognosis is worse (p= 0,0005). Thus suggesting that apoptosis can be used as a prognostic marker in canine breast tumors.

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Published

2003-01-01

Issue

Vol. 40 No. 5 (2003)

Section

UNDEFINIED

License

The journal content is authorized under the Creative Commons  BY-NC-SA  license (summary of the license: https://creativecommons.org/licenses/by-nc-sa/4.0 | full text of the license: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode) 

How to Cite

1.
Pereira KS, Zuccari DAP de C, Cury PM, Cordeiro JA. Apoptosis as a prognostic marker in canine mammary tumors by TUNEL. Braz. J. Vet. Res. Anim. Sci. [Internet]. 2003 Jan. 1 [cited 2024 Apr. 16];40(5):359-65. Available from: https://www.revistas.usp.br/bjvras/article/view/11315