In vitro production of bovine embryo in tubes without gaseous atmosphere control

Authors

  • Mario Kurtz Filho Universidade de Santa Maria, Centro de Ciências da Saúde, Departamento de Morfologia, Santa Maria, RS
  • Mara Iolanda Batistella Rubin Universidade de Santa Maria, Centro de Ciências Rurais, Departamento de Clínica de Grandes Animais, Laboratório de Embriologia Animal, Santa Maria, RS
  • Carlos Antonio Mondino Silva Universidade de Santa Maria, Centro de Ciências Rurais, Departamento de Clínica de Grandes Animais, Laboratório de Embriologia Animal, Santa Maria, RS
  • Lucio Pereira Rauber Universidade de Santa Maria, Centro de Ciências Rurais, Santa Maria, RS
  • Denis Faustino Alves Universidade de Santa Maria, Centro de Ciências Rurais, Santa Maria, RS
  • Manoel Francisco de Sá Filho Universidade de Santa Maria, Centro de Ciências Rurais, Departamento de Clínica de Grandes Animais, Laboratório de Embriologia Animal, Santa Maria, RS
  • José Henrique Souza da Silva Universidade de Santa Maria, Centro de Ciências Rurais, Departamento de Zootecnia, Santa Maria, RS

DOI:

https://doi.org/10.1590/S1413-95962003000300008

Keywords:

Oocytes, Maturation in vitro, Fertilization, Embryos, HEPES

Abstract

The increase in OPU utilization requires an alternative to start the early phases of PIV without a controlled atmosphere. The development of a practical and simple way to transport/mature/fertilize might enhance the efficiency of the laboratory and consequently decrease its production costs. The aim of this study was to develop a method for cumulus oocytes complexes (CCO) maturation and fertilization in polypropylene tubes without atmosphere control kept in water bath. The in vitro maturation performed with controlled atmosphere was in TCM-199 modified (control group). The water bath treatments in tubes the TCM-199 had a addition of 25 mM of N-2-hidroxyethylpiperazine-N'-2-ethanosulfonic acid (HEPES). The CCO in vitro fertilization was performed in Talp-Fert medium with 10mM a 28.7 mM of HEPES, under mineral oil in tubes kept in water bath at 39°C, during 10 to 18 hours. Zygotes were cultured 4 well dishes, in bag system, with SOFaa medium with 5,00% estrus cow serum, under mineral oil, in incubator with 5,00%CO2, 5,00%O2 and 90,00%N2, at 39°C for 9 days. The results showed that CCO can be matured for 24 hours in tubes kept in water bath and also fertilized during 10 hours in tubes with Talp-Fert medium added of 25mM HEPES. The cleavage, D7 and D9 blastocysts rates in tubes without controlled atmosphere were similar (P>;0.05) to those in the incubator procedures. However, the fertilization in Talp-Fert medium added of 10mM a 28.7 mM of HEPES in tubes kept in water bath for 18 hours had a detrimental effect on the embryonic development.

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Published

2003-01-01

Issue

Section

UNDEFINIED

How to Cite

1.
Kurtz Filho M, Rubin MIB, Silva CAM, Rauber LP, Alves DF, Sá Filho MF de, et al. In vitro production of bovine embryo in tubes without gaseous atmosphere control. Braz. J. Vet. Res. Anim. Sci. [Internet]. 2003 Jan. 1 [cited 2024 Jul. 16];40(3):209-16. Available from: https://www.revistas.usp.br/bjvras/article/view/11357